【摘要】 目的:探讨白细胞介素23(IL-23)在变应性鼻炎(AR)患者鼻黏膜中的表达及意义。方法:收集12例AR患者(AR组)下鼻甲黏膜及11例单纯性鼻中隔偏曲患者下鼻甲黏膜(对照组),采用免疫组织化学方法和实时定量RT-PCR方法检测下鼻甲黏膜中IL-23的表达。结果:IL-23p19mRNA在AR组中的表达水平较对照组明显增高(P<0.01)。免疫组织化学显示IL-23蛋白主要表达于黏膜固有层的炎性浸润细胞,AR组鼻黏膜中IL-23阳性细胞数明显高于对照组(P<0.01)。IL-23p19mRNA和蛋白表达水平与黏膜固有层总炎性细胞浸润程度及固有层胶原纤沉积程度成显著正相关(对于mRNA表达水平,相关系数分别为0.678和0.834;对于蛋白表达水平,相关系数分别0.644和0.721,均P<0.01)。结论:IL-23可能参与了AR的慢性炎症和组织重塑。更多还原

【Abstract】 Objective:To investigate the expression of interleukin(IL)-23 in the nasal mucosa of allergic rhinitis patients and its significance.Method:mRNA and protein expression of IL-23 in inferior turbinate mucosa from 12 allergic rhinitis patients and 11 control patients was measured by means of real-time RT-PCR and immunohistochemistry,respectively.Result:IL-23p19 mRNA relative expression level in nasal mucosa was significantly increased in allergic rhinitis patients compared with normal controls(P<0.01).Immunohistochemical staining demonstrated that IL-23 protein was mainly expressed by infiltrating inflammatory cells in lamina propria and there was increased number of IL-23 positive cells in allergic rhinitis patients in comparison with normal controls.Correlation analysis showed that the mRNA and protein expression level of IL-23 was significantly positively correlated with the number of the inflammatory cells(r=0.678 and 0.644,respectively;both P<0.01)and the degree of subepithelial collagen deposition(r=0.834 and 0.721,respectively;both P<0.01).IL-23p19 mRNA relative expression level in nasal mucosa was significantly decreased in allergic rhinitis patients who used glucocorticoids compared with controls(P<0.01).Conclusion:IL-23 may contribute to the chronic inflammation and airway remodelling in allergic rhinitis.更多还原