【摘要】 为探讨全反式维甲酸在人卵巢癌细胞生长和增殖中的作用机制,选用人卵巢癌细胞株HO8910在体外进行培养,培养时添加不同浓度的全反式维甲酸(all-trans retinoic acid,ATRA),作用24h、48h、72h后,采用MTT比色法测定细胞生长抑制率;采用流式细胞术(flow cytometry,FCM)进行细胞周期分析;采用逆转录多聚酶链反应(reverse transcription polymerasechain reaction,RT-PCR)检测维甲酸α受体(retinoic acid receptorα,RARα)mRNA的表达。结果是不同浓度的全反式维甲酸对HO8910细胞生长均起到抑制作用(P<0.05),并呈浓度和时间依赖关系;能够增加G1期细胞比例,同时降低S、G2/M期细胞比例(P<0.05),细胞增殖指数PI?(P<0.05)降低;能够上调维甲酸α受体mRNA的表达(P<0.05),从而抑制卵巢癌细胞增殖。说明全反式维甲酸对卵巢癌细胞HO8910的增殖具有显著的抑制作用,其机制可能是通过上调维甲酸α受体mRNA的表达来实现的。更多还原

【Abstract】 To study the effect of all-trans retinoic acid (ATRA) in the proliferation of human ovarian cancer cells, HO8910 human ovarian cancer cell lines were cultured in vitro and treated with different concentrations of all-trans retinoic acid for 1, 2 or 3 days.Cell proliferation was evaluated by MTT assay.Cell cycle was analayzed by flow cytometry (FCM).The expression of retinoic acid receptor α mRNA was detected by reverse transcription- polymerase chain reaction (RT-PCR).It is resulted that different concentrations of all-trans retinoicacid could inhibit the growth of HO8910 cells(P<0.05) in a time and dose-dependent manner. All-trans retinoic acid increases the rates of G1 and decreases the rates of S and G2/M(P<0.05), and therefore,it decreases cells proliferation index (PI﹡)(P<0.05).It could up-regulate the expression of retinoic acid receptor α mRNA(P<0.05),then inhibit proliferation of HO8910 cells.It is conclused that all-trans retinoic acid could inhibit the proliferation of human ovarian cancer cells HO8910,the molecular basis may be associated with the up-regulated mRNA of retinoic acid receptor α.更多还原