【摘要】 目的通过观察前列腺素(prostaglandin,PG)E2合成通路上各个酶在正常口腔黏膜、口腔黏膜不典型增生、口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达情况,研究PGE2合成通路在OSCC发生过程中的作用。方法收集9例正常口腔黏膜、37例口腔黏膜不典型增生和53例口腔鳞状细胞癌的标本,提取组织中的mRNA,用RT-PCR观察PGE2合成同路上的各参与基因胞质型细胞磷脂酶(cytosolic phospholipase A2,cPLA2)、环氧化酶(cyclooxygenase,COX)-1、COX-2、膜相关前列腺素E合成酶(membrane-associated prostaglandin E synthase,mPGES)-1和mPGES-2的mRNA在三种组织中的表达情况。结果 cPLA2、COX-2和mPGES-1的mRNA在口腔黏膜不典型增生和OSCC中的表达明显高于在口腔正常黏膜中的表达(P<0.01),三者在不典型增生和OSCC中的表达无显著差异(P>0.05)。COX-1和mPGES-2的mRNA在三种组织中的表达无明显差异(P>0.05)。结论 cPLA2-COX-2-mPGES-1作为PGE2的合成通路可能参与调控了OSCC的发生,提示cPLA2和mPGES-1可以作为OSCC化学治疗的新的分子靶标。更多还原

【Abstract】 Objective To investigate the expression of each candidate genes of PGE2 biosynthesis pathway in normal,atypical hyperplasia and squamous carcinoma of oral mucosa. Methods 9 tissue samples of normal oral epithelium,37 cases of oral atypical hyperplasia and 53 cases of oral squamous cell carcinoma(OSCC)were collected. The levels of cytosolic phospholipase A2(cPLA2),cyclooxygenase(COX)-1,COX-2,membrane-associated prostaglandin E synthase (mPGES)-1 and mPGES-2 mRNA in three tissues were evaluated by RT-PCR. Results The levels of cPLA2,COX-2 and mPGES-1 mRNA were much higher in atypical hyperplasia tissues and OSCCs than in normal tissues (P<0.01),which didn’t have significant difference between atypical hyperplasia tissues and OSCCs(P>0.05). On the other hand,there was no difference of COX-1 and mPGES-2 expression among three tissues (P>0.05). Conclusions COX-2 and cPLA2,key factors in PGE2 biosynthesis,worked in concert to promote the progression of normal oral epithelium toward oral carcinoma. cPLA2-COX-2-mPGES-1 signal pathway may play an essential role in promoting oral carcinogenesis.更多还原