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丙醇-硫酸铵双水相体系与超声集成提取旱莲草总黄酮的研究
On Extraction of General Flavonoids from Eclipta Alba with Propyl Alcohol and Ammonium Sulfate Aqueous Two-phase Separation and Ultrasonic Integration
【摘要】 将超声波技术和双水相萃取技术耦合用于提取旱莲草中总黄酮,通过单因素实验得出最佳提取条件为:硫酸铵用量120 mg/mL,丙醇50%(体积分数),料液比1∶30,超声提取30 min,此时总黄酮平均得率为4.22%,优于加热回流提取。
【Abstract】 Ultrasonic extraction technology,integrated with propyl-alcohol and ammonium sulfate aqueous two-phase separation was used to extract general flavonoids from Eclipta alba.The optimum extraction conditions from single-factor experiment were: the amount of ammonium sulfate was 12 %(W %);propyl-alcohol occupied 50 %;mass and liquidratio was 1∶30 and ultrasonic extraction was 30min.Then,the average yield of general flavonoids was 4.22 %,which was better than that from heating reflux extraction.
【关键词】 旱莲草;
双水相;
超声提取;
总黄酮;
【Key words】 Eclipta alba; aqueous two-phase; ultrasonic extraction; general flavonoids;
【Key words】 Eclipta alba; aqueous two-phase; ultrasonic extraction; general flavonoids;
- 【文献出处】 昆明冶金高等专科学校学报 ,Journal of Kunming Metallurgy College , 编辑部邮箱 ,2010年05期
- 【分类号】TQ461
- 【被引频次】7
- 【下载频次】255