【摘要】 目的:建立双花解毒合剂中黄芩苷高效液相色谱(HPLC)测定方法。方法:采用高效液相色谱法,色谱柱Kromasil C18(250mm×4.60mm,5μm);以甲醇-0.4%磷酸水(52∶48)为流动相,流速为1mL/min,检测波长为280nm。结果:黄芩苷的线性范围为0.056～0.556μg/mL,r=0.9999,平均加样回收率(n=6)为101.40%,RSD为2.40%。结论:本法简便可行、重复性好,可用于双花解毒合剂中黄芩有效成分的含质量测定。更多还原

【Abstract】 Objective:To establish the method for determination of effective components in Shuanghua Jiedu mixture by HPLC.Methods:The contents of baicalin were determined by a HPLC system using following conditions:the column was Kromasil C18 (250mm×4.60mm,5 μm),CHOH-0.4% H3PO4water solution(52:48) was used as mobile phase,the flow rate was 3 1 mL/min and the detection wavelength was at 280 nm.Results:The linear range of hydroxysafflor yellow A was 0.056～ 0.556 μg/mL,r =0.999 9,its average recovery (n = 6) was 101.40% and RSD was 4.18%.Conclusion:The results indicate that the HPLC method is simple,accurate,highly selective and reproducible,thus it could be used as quality control of compound Shuanghua Jiedu mixture.更多还原