【摘要】 目的研究不对称二甲基精氨酸对THP-1源性巨噬细胞表达巨噬细胞移动抑制因子的影响。方法 150nmol/L佛波酯诱导THP-1细胞48h使其转化为巨噬细胞,并用抗人CD68进行免疫细胞化学鉴定,以不同浓度的不对称二甲基精氨酸(1、5、10、20和30μmol/L)作用于THP-1源性巨噬细胞24h及20μmol/L不对称二甲基精氨酸作用于THP-1源性巨噬细胞0h、6h、12h、24h及48h,逆转录聚合酶链反应检测各组细胞的巨噬细胞移动抑制因子mRNA表达水平,酶联免疫吸附法检测各组细胞上清液中巨噬细胞移动抑制因子的蛋白含量。结果与对照组相比,5、10、20和30μmol/L的不对称二甲基精氨酸作用于THP-1源性巨噬细胞24h后,巨噬细胞移动抑制因子的mRNA和蛋白表达水平均明显提高(P<0.05)。与0h组相比,20μmol/L不对称二甲基精氨酸作用于THP-1源性巨噬细胞12、24和48h,巨噬细胞移动抑制因子的mRNA和蛋白表达水平均明显提高(P<0.05)。结论不对称二甲基精氨酸可上调THP-1源性巨噬细胞的巨噬细胞移动抑制因子表达,并呈剂量和时间依赖性,不对称二甲基精氨酸可能通过诱导巨噬细胞移动抑制因子的表达而促进动脉粥样硬化的发生和发展。更多还原

【Abstract】 Aim To investigate the effects of asymmetric dimethylarginine(ADMA)on the expression of macrophage migration inhibitory factor(MIF)in THP-1 monocyte-derived macrophage cells.Methods After induced by 150 nmol/L phorbol 12 myristate 13 acetate(PMA)for 48 h,THP-1 monocyte cells differentiated into macrophage cells.And THP-1 monocyte-derived macrophage cells were identified by immunocytochemistry with anti-human CD68.After THP-1 monocyte-derived macrophage cells were incubated with ADMA of different concentrations(1 μmol/L,5 μmol/L,10 μmol/L,20 μmol/L,30 μmol/L)for 24 h and 20 μmol/L ADMA for 0 h,6 h,12 h,24 h and 48 h,the expressions of MIF in mRNA and protein were measured by means of RT-PCR and enzyme-linked immunosorbent assay respectively.Results Treatments of THP-1 macrophage cells with 5 μmol/L,10 μmol/L,20 μmol/L,30 μmol/L ADMA for 24 h resulted in an increase in the mRNA and protein levels of MIF.Treatments of THP-1 macrophage cells with 20 μmol/L ADMA for 12 h,24 h,48 h,resulted in an increase in the mRNA and protein levels of MIF.Conclusion ADMA upregulated the expression levels of MIF in cultured THP-1 monocyte-derived macrophage cells in a dose-and time-dependent manner,which may result in the atherosclerosis.更多还原