【摘要】 目的观察大鼠脑缺血再灌注后凋亡分子C/EBP同源蛋白的表达变化,探讨该分子对神经细胞凋亡的影响。方法制备SD大鼠大脑中动脉闭塞模型,逆转录聚合酶链反应法、免疫组织化学染色分别测定大鼠脑缺血半暗带区再灌注后不同时相C/EBP同源蛋白mRNA及蛋白的表达变化;缺口末端标记法测定神经细胞凋亡。结果模型组C/EBP同源蛋白mRNA表达于再灌注后12h达高峰,其蛋白表达于再灌注后24h达高峰,与神经细胞凋亡变化趋势相平行。结论大鼠脑缺血再灌注可诱导C/EBP同源蛋白表达,C/EBP同源蛋白在缺血再灌注所致神经细胞凋亡中可能发挥重要作用。更多还原

【Abstract】 Aim To detect the expression development of proapoptic molecule C/EBP homology protein(CHOP)mRNA and protein,and explore its effect on neuronal apoptosis after cerebral ischemia/reperfusion in rats.Methods Transient focal cerebral ischemia was induced by middle cerebral artery occlusion(MCAO)for 2 hours followed by reperfusion in sprague-dawley rats.Then,the expression of CHOP protein and/or mRNA were measured with methods of immunohistochemistry and reverse transcription-polymerase chain reaction(RT-PCR)at 1 h,3 h,6 h,12 h and 24 h after reperfusion in cerebral cortex of rats.The neuronal apoptosis was detected by the method of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL).Results In cerebral ischemia model group,the expression level of CHOP mRNA reached a peak at 12 h after reperfusion and that of CHOP protein reached a peak at 24 h after reperfusion,which paralleled with the tendency of neuronal apoptosis development.Conclusion Cerebral ischemia reperfusion may induce CHOP expression.CHOP may play an important role in neuronal apoptosis induced by cerebral ischemia/reperfusion.更多还原