【摘要】 目的实验观察生化指标检测过程中可能出现测试项目间的交叉污染对日立7180全自动生化分析仪使用的影响。方法对24个常规生化项目丙氨酸氨基转移酶(ALT)、碱性磷酸酶(AKP)、谷氨酰氨基转移酶(GGT)、总胆汁酸(TBA)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、总蛋白(TP)、清蛋白(ALB)、前清蛋白(PA)、总胆红素(TBIL)、直接胆红素(DBIL)、葡萄糖(GLU)、果糖醇(FTS)、尿酸(UA)、尿素氮(BUN)、肌酐(CRE)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、磷(P)、钙(Ca)、天冬氨酸转氨酶(AST)、胆碱酯酶(CHE)进行交叉污染试验观察。结果 24个项目交叉污染试验结果显示,FTS施于CHE、PA的污染,CHOL、TG施于TBA的污染,HDL-C施于TG的污染均可明显影响其测定结果。结论实际使用全自动生化仪随机组合项目时,应注意项目位置的特别设置,某些项目检测数据异常,应预设清洗程序进一步清洗。对可能引起结果改变的因素具体观察分析,并采取相应的控制措施,为临床提供科学、准确的诊疗依据。更多还原

【Abstract】 Objective To study the cross-contamination of Hitach 7180 automatic biochemical analysis instrument.Methods Put common biochemistry test onto ALT,AKP,GGT,TBA,LDH,CK,TP,ALB,PA,TBIL,DBIL,GLU,FTS,UA,BUN,CRE,CHOL,TG,HDL-C,LDL-C,P,CA,AST,and CH,at the same time,the cross-contamination of different biochemistry test was measured and analyzed.Results Among 24 cross-contamination tests,the test of FTS has significant influence in the test of CHE and PA.CHOL and TG have significant influences on the test of TBA,at the sone time,the test of HDL-C has a significant influence on the test of TG.Conclusion The cross-contamination result make it clear that some abnormal data came out because of the automatic biochemical analysis instrument’s random assessment tests.It is necessary to set up a washing program in advance and change the position of the cross-contamination.Generally speaking,all of the factors influencing the test value should be observed and analyzed.The measures avoiding these factors should be taken rapidly,so that it can provide the clin:cal doctor with the scientific and pvesise diagnose and evidence.更多还原