【摘要】 利用椰子成熟胚和幼花序研究了愈伤组织诱导和控制褐变的方法。结果表明:椰子幼花序比成熟胚诱导愈伤组织的速度快且效果好。在幼花序愈伤组织的诱导中,以佛焰苞长度为16.5 cm的幼花序诱导效果最好。在Y3培养基中添加浓度为25 mg/L的2,4-D时,愈伤组织的诱导率最高。加入2.5 g/L活性炭和5 g/L的聚乙烯吡咯烷酮(PVP)对于培养物褐变有良好的抑制作用。更多还原

【Abstract】 The mature embryo and immature inflorescence of coconut were used to study the callus inducement and brown-turning prevention.The results showed that immature inflorescence as explant achieved faster and better results of callus inducement than the mature embryo did.When immature inflorescence was used as explant,the immature inflorescence with 16.5 cm long spathe had the best effect for callus inducement.The rate of callus inducement was the highest in Y3 medium containing 25 mg/L 2,4-D.Adding 2.5 g/L active carbon and 5 g/L PVP into the medium was good for the inhibition of brown-turning.更多还原