【摘要】 目的探讨肿瘤坏死因子-α(TNF-α)诱导大鼠骨髓间充质干细胞向神经细胞分化的作用。方法采用密度梯度离心法分离rMSCs,取培养至3～5代的rMSCs以含TNF-α 5ng/ml的培养基培养,镜下观察细胞诱导后形态的变化,采用免疫荧光细胞化学染色法检测神经前体细胞标志物巢蛋白nestin和神经元特异性烯醇化酶在细胞中的表达强度和分布。结果rMSCs经TNF-α诱导6h后基本表现为典型神经样细胞形态,nestin和NSE呈阳性表达。结论TNF-α具有体外诱导骨髓间充质干细胞向神经样细胞分化的作用。更多还原

【Abstract】 Objective To investigate whether TNF-α can induce neuron-like cells formation from rat marrow stromal cells(rMSCs) in vitro.Methods rMSCs were isolated from bone marrow by density gradient centrifugation with Fieoll-Paque and cells between passeges 3～5 were induced by TNF-α at 5ng/ml.The cellular morphological changes of induced rMSCs were observed by microscope and we monitored expression of nestin and neuronal specific enolase(NSE) in rMSC differention by indirect immuno-fluorescent cytochemistry staining.Results After treated with TNF-α in 6 hours,rMSCs showed obviously morphological changes and expressed the protein for nestin and NSE.Conclusion TNF-α can induce rMSCs into neuron-like cells.更多还原