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大花石榴胚培养再生植株
Plantlet Regeneration from the Embryo of Punica granatum cv. Nana in vitro
【摘要】 以大花石榴的胚为外植体在MS+BA1+NAA0.2培养基中诱导愈伤并产生不定芽,在MS+BA0.5+NAA0.2培养基中进行芽苗的增殖培养,在1/2MS+NAA0.5+IBA0.5培养基中诱导芽苗生根。试管苗在珍珠岩基质中罩塑料杯保湿炼苗后即可移栽入土中。
【Abstract】 The embryos of Punica granatum cv. Nana were used as explants to initiate calluses in the medium of MS+BA1+NAA0.2.The calluses with small buds were cultured in the medium of MS+BA0.5+NAA0.2 for the multiplication of sprouts.The shoots regenerated were rooted in the medium of 1/2MS+ NAA0.5+IBA0.5.The seedlings covered with plastic cups were tamed in the medium of pearlite for a short period and transplanted into soil as the terminal buds had sprouted.
【关键词】 组织培养;
大花石榴;
胚;
再生植株;
【Key words】 Tissue culture; Punica granatum cv. Nana; Embryo; Plantlet regeneration;
【Key words】 Tissue culture; Punica granatum cv. Nana; Embryo; Plantlet regeneration;
- 【文献出处】 江西科学 ,Jiangxi Science , 编辑部邮箱 ,2010年02期
- 【分类号】S665.4
- 【被引频次】6
- 【下载频次】105