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大花石榴胚培养再生植株

Plantlet Regeneration from the Embryo of Punica granatum cv. Nana in vitro

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【作者】 闵炜陈志萍

【Author】 MIN Wei,CHEN Zhi-ping(Shanghai Vocational Technical College of Agriculture &Forestry,Shanghai 201600 PRC)

【机构】 上海农林职业技术学院

【摘要】 以大花石榴的胚为外植体在MS+BA1+NAA0.2培养基中诱导愈伤并产生不定芽,在MS+BA0.5+NAA0.2培养基中进行芽苗的增殖培养,在1/2MS+NAA0.5+IBA0.5培养基中诱导芽苗生根。试管苗在珍珠岩基质中罩塑料杯保湿炼苗后即可移栽入土中。

【Abstract】 The embryos of Punica granatum cv. Nana were used as explants to initiate calluses in the medium of MS+BA1+NAA0.2.The calluses with small buds were cultured in the medium of MS+BA0.5+NAA0.2 for the multiplication of sprouts.The shoots regenerated were rooted in the medium of 1/2MS+ NAA0.5+IBA0.5.The seedlings covered with plastic cups were tamed in the medium of pearlite for a short period and transplanted into soil as the terminal buds had sprouted.

  • 【分类号】S665.4
  • 【被引频次】6
  • 【下载频次】105
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