【摘要】 目的探讨孕酮对子宫巨噬细胞数量及其膜受体CD14、CD204表达的影响,以及孕酮和巨噬细胞在子宫局部免疫中的作用。方法 1.25只正常非孕鼠随机分为空白对照组、阴性对照组和低、中、高3个不同浓度孕酮处理实验组,每组5只。低、中、高浓度实验组每天分别皮下注射0.4mg、2.0mg、4.0mg孕酮,阴性对照组注射等体积芝麻油,连续注射5d;空白对照组不做任何处理。2.孕4d和5d小鼠各15只,分别随机分为空白对照组、阴性对照组和实验组,每组5只。实验组分别于当天早晨皮下注射4mg孕酮,阴性对照组注射等体积芝麻油,空白对照组不做任何处理。以上各组均于末次注射后6h取子宫,通过非特异性酯酶(α-NAE)染色和免疫组织化学检测子宫α-NAE+、CD14+、CD204+巨噬细胞的变化。结果与对照组相比,注射孕酮后,非孕鼠子宫内膜、肌层和外膜的α-NAE+及CD14+巨噬细胞极显著减少(P<0.01),并与剂量呈正相关,但未检测到CD204+巨噬细胞;孕4d、5d小鼠子宫α-NAE+、CD14+、CD204+巨噬细胞均显著增多(P<0.01)。结论孕酮可通过调节巨噬细胞数量、分布及膜受体的表达影响子宫局部免疫状态,替代途径活化的巨噬细胞可能介导了妊娠期母胎免疫耐受的维持。更多还原

【Abstract】 Objective To study the influence of progesterone on the number of the mouse uterine macrophages and the expression of its membrane receptors CD14 and CD204,and to explore the role of pro-gesterone and macrophage in uterus regional immunity.Methods 1.Twenty-five non-pregnant and healthy mice were randomly and evenly divided into blank control group,negative control group and experimental group which divided into low,medium and high progesterone concentration subgroups in advance and every group had 5 mice.The progesterone at the doses of 0.4mg,2.0mg,and 4.0mg were intracutaneously injected into the mice of the experimental group,respectively,while the same doses of placebo were injected into the negative control group in the same condition and both were injected continuously for 5 days;the blank control group was stayed intact.2.Gestational day 4 to 5 mice,the 15,were also divided into blank control,negative control and experiment groups randomly,5 mice in each group.The progesterone at the dose of 4mg was intracutaneously injected into the experimental mice in the morning,and as well the same doses of placebo were injected into the negative control group mice;the blank control group was left intact.All of the above mice uteri were collected 6 hours later after the final injection.Non-specific esterase(α-NAE) staining and immunohistochemistry methods were used to identify the varieties of α-NAE +,CD14 +,CD204 + macrophages in the uterus.Results Compared with the negative control group,after injecting progesterone,the amount of the uterine macrophages with positive CD14 receptor immunohistochemical staining were significantly decreased with dose increasing in the non gestational group.For the gestational day 4 to day 5 mice,the amounts of the uterine macrophages with the positive CD14 and CD204 receptors immunohistochemical staining were significantly increased(P < 0.01).Conclusion Progeterone can affect the regional immunity of uterus by adjustingthe quantity of macrophages or its distribution or the expression of its membrane receptor.And the macrophages from alternative activation pathway might have a contribution to the maintenance of the maternofetal immune tolerance.更多还原