【摘要】 目的观察尼古丁处理大鼠脑内多巴胺转运体(DAT)和酪氨酸羟化酶(TH)的表达变化,探讨尼古丁处理对大鼠脑内多巴胺(DA)能神经体系的影响。方法选用雄性Wistar大鼠按每日0.4mg/kg腹腔注射尼古丁7d;利用免疫组织化学和免疫印迹法,检测尼古丁处理大鼠有关脑区DAT和TH的表达改变。结果与对照组相比:1.免疫组织化学显示,尼古丁处理组大鼠伏核(NACC)和腹则被盖区(VTA)的DAT灰度值降低了12.43%和12.85%;TH的灰度值则降低了11.87%和10.09%。2.免疫印迹法显示,尼古丁处理组大鼠尾壳核(CPu)-NACC、黑质(SN)-VTA的DAT与β-肌动蛋白(β-actin)条带相对吸光度比值增加了75.68%和117.14%;而TH的比值则分别增加了66.32%和60.31%。结论尼古丁处理增加大鼠脑内DAT和TH的表达,这可能与尼古丁的成瘾机制有关。更多还原

【Abstract】 Objective To study the effect of nicotine on the dopaminergic system by analyzing the altered expression of dopamine transporter(DAT) and tyrosine hydroxylase(TH) in the brain of nicotine-treated rats.Methods Healthy male Wistar rats were selected and then injected for 7 days with 0.4 mg/kg nicotine every day.Immunohistochemistry and Western blotting were used to detect the altered expression of DAT and TH in the dopaminergic system in different brain regions of the nicotine treated rats.Results Compared with the control:1.Immunohistochemistry showed the mean gray value of DAT in nucleus accumbens (NACC) and ventral tegmental area(VTA) decreased 12.43 % and 12.85 % respectively,the mean gray value of TH in NACC and VTA decreased 11.87 % and 10.09 % .2.Western blotting showed the ratio of relative absorbance of DAT/β-actinin in CPu-NACC and SN-VTA of the the nicotine-treated rats increased 75.68 % and 117.14 % respectively; the ratio of relative absorbance of TH/β-actinin in caudate putamen (CPu)-NACC and substantia nigra ( SN)-VTA increased 66.32 % and 60.31 % respectively.Conclusion Nicotine treatment increases the expression of DAT and TH in rat brain,which may have some relations with the mechanism of nicotine dependence.更多还原