【摘要】 目的探讨睡眠剥夺后大鼠皮质、海马和杏仁核微管相关蛋白-2(MAP-2)及神经丝蛋白(NF-200)表达的变化。方法采用改良小平台水环境法制作大鼠睡眠剥夺模型,大鼠随机分为睡眠剥夺组(SD组)、环境对照组(TC组)和空白对照组(CC组)。SD组包括睡眠剥夺6h、12h、1d、2d、3d、5d、7d共7个时点,每个时间点5只大鼠,CC组5只。免疫组织化学法观察MAP-2和NF-200阳性表达。结果睡眠剥夺5d和7d时,皮质、CA1、CA2、CA3、齿状回和杏仁核MAP-2和NF-200阳性表达减少(P<0.05,P<0.01)。结论睡眠剥夺可导致脑组织MAP-2和NF-200表达减少。更多还原

【Abstract】 Objective To study the expression of microtubule-associated protein-2(MAP-2) and neurofilament-200(NF-200) in cortex,hippocampus and amygdaloid body of rat after sleep deprivation(SD).Methods SD model was made by modified multiple platform method(MMPM),rats were divided into sleep deprivation group(SD),tank control group(TC),cage control group(CC).The sleep deprivation group included seven time-length groups(sleep deprivation 6 hours,12 hours,1 day,2 days,3 days,5 days and 7 days).Five rats were assigned to each group.The expression of MAP-2 and NF-200 were detected by immunohistochemical method.Results At the 5th day and 7th day,we found that the density of MAP-2 and NF-200 positive neuron decreased in cortex,CA1,CA2,CA3,dentate gyrus and amygdaloid body(P < 0.05,P < 0.01).Conclusion SD can induce reduction of expression of MAP-2 and NF-200.更多还原