【摘要】 目的探讨脂多糖(LPS)对小鼠肝脏和脾脏铁代谢相关基因mRNA水平表达的影响。方法10只2月龄雄性小鼠腹腔注射LPS(0.5μg/g),6h后处死并取血液、肝脏和脾脏组织进行血常规测定,使用试剂盒检测血清铁和总铁结合力;使用半定量RT-PCR检测小鼠肝脏和脾脏铁代谢相关基因(HP)、膜铁转运蛋白1(Fpn1)和转铁蛋白受体1(TfR1)以及脾脏白细胞介素-6(IL-6)的mRNA变化。结果注射LPS6h后可造成小鼠脾脏IL-6表达增加,肝脏HP表达增加(P<0.05),肝脏和脾脏Fpn1表达减少,而肝脏TfR1表达亦减少,血清铁下降和适度贫血(P<0.05)。结论LPS可通过影响肝脏和脾脏铁相关基因HP、Fpn1和TfR1的mRNA表达而影响血清铁状态。更多还原

【Abstract】 Objective To investigate the effects of lipopolysaccharide(LPS) on mRNA expression of iron metabolism related genes.Methods Ten male mice(2 months) were injected intraperitoneally with lipopolysaccharide(0.5 μg/g).After 6 hours,mice were sacrificed and then sera,liver and spleen were collected.The mice blood routine was measured.The serum iron and total iron binding capacity(TIBC) were determined with reagent kit.The quasiquantitative reverse transcription polymerase chain reaction(RT-PCR) was performed for mRNA of hepatic hepcidin(HP), ferroportin1(Fpn1),transferrin receptor 1(TfR1) and spleenic HP,Fpn1 and interleukin-6(IL-6).Results The serum iron and TIBC were reduced in mice injected LPS,which exhibited mild anemia(P < 0.05 ) .LPS can increase the expression of hepatic hepcidin and decrease Fpn1 and TfR1 in liver after LPS administration 6 hours(P < 0.05 ) .In spleen,IL-6 was upregulated and Fpn1 downregulated(P < 0.05) .Conclusion LPS can influence serum iron through regulating the mRNA expression of hepatic and spleenic iron metabolism related genes,such as HP,Fpn1 and TfR1.更多还原