【摘要】 目的探讨17-β雌二醇治疗脊髓损伤的分子机制,为临床应用雌激素治疗脊髓损伤提供理论依据。方法成年雄性SD大鼠180只,采用改良的Allen法构建大鼠急性脊髓挫伤模型后经17-β雌二醇治疗,采用化学比色法测定脊髓组织中丙二醛(MDA)和内源性巯醇抗氧化物(酶):还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)含量;免疫组织化学分析凋亡相关因子Caspase-3、Bcl-2和Bax蛋白的表达变化。结果17-β雌二醇治疗组与脊髓损伤组比较,BBB评分明显增加;在多个时间点MDA含量明显降低;巯醇抗氧化物(酶)GSH、SOD和GSH-Px含量显著增加;Caspase-3和Bax表达的阳性细胞数明显减少;Bcl-2表达的阳性细胞数明显增加(P<0.05)。结论大剂量的17-β雌二醇治疗可能通过调控内源性巯醇抗氧化物(酶),提高肢体运动功能,抑制细胞凋亡,从而减轻脊髓损伤程度。更多还原

【Abstract】 Objective To examine the protective effects of 17-β estradiol on the experimental model of spinal cord injury(SCI) rats.Methods One hundred and eighty male Sprague Dawley(SD) rats,after Allen’s model,SD rats were divided into three groups:the sham group,the acute spinal cord injury(control groups) and the acute spinal cord injury supplying with 17-β estradiol treatment group.SCI was made by Allen ’s weight dropping,impacting on the posteriors of spinal cord T10.The content of malonyldialdehyed(MDA ),glutathione(GSH ),superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) were determined by chromatometry.The expressions of Caspase-3 and Bcl-2 family in the injured spinal cord were detected by immunohistochemical staining.Results The BBB scores at each time point in 17-β estradiol treatment group were significantly higher than that in SCI group(P < 0.05).The contents of GSH, SOD,GSH-Px and the expression of Bcl-2 protein at the majority of time point in 17-β estradiol treatment group were significantly higher than that in SCI group(P < 0.05),however,the MDA,Caspase-3 and Bax were markedly decreased(P < 0.05).Conclusions This study suggests that 17-β estradiol administration might prevent the cells from SCIinduced apoptosis by triggering to reduce the oxidative stress.更多还原