【摘要】 目的:观察慢性盐负荷对自发性高血压大鼠(SHR)胸主动脉TGFβ1表达的影响,探索盐在心血管疾病发生发展中的作用机制。方法:85周龄SHR雄性大鼠16只,高盐组(4%NaCl)、正常盐组(0.4%NaCl)各8只,运用鼠尾动脉测定大鼠血压,于饲养4周处死,取胸主动脉行HE染色进行大体病理形态学观察,采用免疫组织化学方法观察主动脉管壁TGFβ1的蛋白表达。结果:4周后高盐组的收缩压(158±13.6)mmHg(1mmHg=0.1333kPa)高于正常盐组(145±10.9)mmHg,两组比较差异有统计学意义(P<0.05);高盐组胸主动脉TGFβ1蛋白表达水平均高于正常盐组,两组比较差异有统计学意义(P<0.05)。结论:慢性盐负荷可使SHR胸主动脉管壁增厚,TGFβ1蛋白表达水平升高。更多还原

【Abstract】 Objective To observe the effect of chronic salt loading on the expression of TGFβ 1 in thoracic aorta of spontaneously hypertensive rats.Methods 16 male SHR rats of 5 weeks old were randomly and averagely divided into high salt(4%NaCl,n=8) and normal salt(0.4%NaCl,n=8) group.The rats were sacrificed after 4 weeks which aortics were collected aorta respectively and dyed with HE for observe general morphology.The protein expression of TGFβ1 were measured by immunohistochemistry.Results After 4 weeks of high salt group,systolic blood pressure(158±13.6) mm Hg higher than the normal salt group(145±10.9) mm Hg,the difference was statistically significant(P<0.05) ;the main chest high salt group artery TGFβ1 protein were higher than the normal salt group,the difference was statistically significant(P<0.05) . Conclusion High-salt dietary could aggravate the vascular remodeling and increasing the expression of TGFβ1 protein.更多还原