【摘要】 利用发根农杆菌A4菌株在西洋参根外植体上直接诱导产生发根。在1/2 MS和MS固体培养基上得到西洋参发根,并在B5液体培养基上建立起发根离体培养系,经连续多代的培养,发根仍保持旺盛生长状态。PCR扩增结果表明,发根农杆菌Ri质粒的rolC基因已整合到西洋参发根基因组中并得到表达。利用紫外分光光度法测得西洋参发根的总皂苷含量达3.88%。经对发根比生长速率的测定,确定1/2 B5培养基(15 g/L蔗糖)、摇床转速100 r/min、每4周更换1次培养基并继代培养1次为西洋参发根生长适宜条件。更多还原

【Abstract】 Hairy roots of Panax quinquefolium L.were induced from the root explants of two-year old Panax quinquefolium L.by Agrobacterium rhizogenes A4 with direct inoculation,the cultured clones of the hairy roots were established on solid 1/2 MS and MS mediums.After several times of subcultures,the hairy roots still maintained vigorous growth.After the determination of UV-spectrophotometry,the total ginsenosides content in the hairy root came up to 3.88%.The suitable culture conditions for Panax quinquefolium L.hairy roots growing were 1/2 B5 liquid medium(15 g/L glucose),in a shaker at 100 r/min,changing the culture solution and subculturing the hairy roots every 4 weeks.更多还原