【摘要】 为了探索脂类分子结构的变化与细胞癌变的关系,应用共聚焦显微拉曼光谱系统,检测结直肠癌组织及邻近正常组织的拉曼光谱,得到归属于脂类CH₂对称伸缩振动在2857 cm^-1和反对称伸缩振动在2891 cm^-1的拉曼峰以及归属于脂类和蛋白C—H伸缩振动在2938 cm^-1的拉曼峰。实验结果显示,结直肠癌组织和邻近正常组织的拉曼光谱存在以下显著的差异:1)与正常组织相比,结直肠癌组织中位于2857 cm^-1和2891 cm^-1的拉曼峰强度相对较弱,而2938 cm^-1拉曼峰的强度相对较强。2)结直肠癌组织中在2891 cm^-1和2857cm^-1的拉曼峰强度比值I₂₈₉₁/I₂₈₅₇小于正常组织的比值。用SPSS16.0统计学软件中平均值之间的比较功能计算出正常组织的I₂₈₉₁/I₂₈₅₇平均值为2.21,癌变组织的I₂₈₉₁/I₂₈₅₇平均值为1.69。研究结果表明,当正常细胞转变为癌细胞时,脂类分子的侧向堆积程度下降,细胞膜流动性增加,脂类与蛋白的相互作用增强,揭示了脂类分子伸缩振动的拉曼光谱与细胞癌变的关系。更多还原

【Abstract】 The purpose of this study is to investigate a relationship between the structure of lipid and colorectal carcinogenesis at the molecular level.Colorectal cancer specimens and adjacent normal tissues were measured using confocal Raman microscopy system.The bands at 2857 cm^-1 and 2891 cm^-1 originate from CH₂ symmetric and asymmetric stretching vibrations,respectively.The band at 2938 cm^-1 arises from symmetric C—H stretching vibrations.The results show that there are prominent differences between colorectal cancer specimens and adjacent normal tissues in Raman spectroscopy:1) The intensity of the bands of the cancer tissue spectrum at 2857 cm^-1 and 2891 cm^-1 are reduced compared with those of the normal spectra.The relative intensity of the band at 2938 cm^-1 of lipid of colorectal cancer is stronger than that in normal tissues.2) The average ratio(I₂₈₉₁/I₂₈₅₇) values is reduced compared with those of the normal spectra.The values for normal and cancerous tissues are 2.21 and 1.69 by the function of ’compare means’ of SPSS16.0 software.The research results demostrated that the lateral packing of lipids is loosened which may result in the fluidity of lipid to be increased and the interactions of lipid and protein increase when normal cells are transformed into cancerous cells.Our data suggested there is the relationship between the stretch vibrations of lipids and colorectal carcinogenesis.更多还原