节点文献

激光扫描共聚焦显微镜在骨髓基质细胞移植中的应用

Application on transplantation of bone marrow stromal cells with Laser scanning confocal microscope

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 万建美罗蔚锋李厚怀包仕尧刘春风张志琳李冰燕

【Author】 WAN Jian-Mei1,LOU Wei-feng2,LI Hou-huai3,BAO Shi-rao2,LIU Chun-feng2,Zhang Zhi-lin2,LI Bing-yan1(1.Center Laboratory of Radiation Medical and Protection of Soochow University,Suzhou 215123,China;2.Second Affiliated Hospital of Soochow University,Suzhou 215007,China;3.First Affiliated Hospital of Soochow University,Suzhou 215007,China)

【机构】 苏州大学放射医学与辐射防护重点实验室苏州大学附属第二医院苏州大学附属第一医院

【摘要】 目的:探讨激光扫描共聚焦显微镜(laser scanning confocal microscope,LSCM)技术在骨髓基质细胞(Bone Marrow Stromal Cells,BMSCs)移植中的应用。方法:使用活细胞示踪剂CM-DiI、细胞核探针DAPI和DNA合成前体物BrdU体外标记BMSCs,移植到动物模型体内,利用LSCM技术,观察BMSCs在受体组织内的分布和分化。结果:体外标记BMSCs后再移植到受体,使用LSCM技术可以观察到BMSCs定位于受体心肌组织和脑组织,并有部分BMSCs表达相应的组织特异性蛋白Cx43和GFAP。结论:利用LSCM技术观察BMSCs在受体组织内分布和分化是一种简单可行的方法。

【Abstract】 Objective:Location and differentiation of bone marrow stromal cells(BMSCs) were investigated with Laser scanning confocal microscope(LSCM).Methods:After BMSCs in pig and rats were loaded with Cell-Tracker CM-DiI,DAPI and BrdU, they were transplantated into animal model,respectively.Location and differentiation of BMSCs in recipitent was observed with LSCM.Result:Location of BMSCs in recipient tissues was detected.Furthermore,BMSCs could differentiate into myocardial cell or glial cells with expression of Cx43 or GFAP in every group.Conclusion:It is a simple and effective method to observe the Location and differentiation of BMSCs with LSCM.

【关键词】 激光扫描共聚焦显微镜骨髓基质干细胞移植
【Key words】 LSCMBMSCstransplantation
  • 【分类号】R318.51;TN249
  • 【被引频次】1
  • 【下载频次】157
节点文献中: 

本文链接的文献网络图示:

本文的引文网络