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正交实验优选月腺大戟根总黄酮的提取工艺
Optimization of Extraction Process of Total Flavones from the Roots of Euphorbiae bracteolata Hayata by Orthogonal Design
【摘要】 目的:研究月腺大戟根中总黄酮类化合物的提取工艺,为研发新型植物源农药提供理论基础。方法:采用索氏提取法对月腺大戟根总黄酮进行提取,以乙醇体积分数、提取时间、提取温度、料液比为实验因素,以提取的总黄酮含量为指标,对提取工艺进行考察,确定最佳提取条件。结果和结论:根总黄酮提取的优化条件为:提取时间2 h,提取温度70℃,料液比1:40,乙醇浓度50%;影响提取的主次顺序为:料液比>提取时间>乙醇浓度>提取温度;其中料液比和提取时间是影响根总黄酮提取效果的显著因素。
【Abstract】 Objective:To study the extraction process of total flavones from the roots of Euphorbiae bracteolata Hayata, and accordingly provide evidence for research and development of botanical pesticide.Method:The total flavones were extracted by Soxhlet way,The experimental factors include;concentration of ethanol,time and temperature of extracting, ratio of material to fluid.The extraction conditions were determined by the content of total flavones.Result and conclusion:The optimal conditions on total flavones extracted of Euphorbiae bracteolata Hayata were as follows:time of extracting is 2 h,temperature of extracting is 70 ℃,ratio of material to fluid is 1:40 and concentration of ethanol is 50 %.The order on purification is ratio of material to fluid > time of extracting > concentration of ethanol > temperature of extracting and the significantly factors are ratio of material to fluid and time of extracting.
【Key words】 Euphorbiae bracteolata Hayata; total flavones; orthogonal design; extraction process;
- 【文献出处】 激光生物学报 ,Acta Laser Biology Sinica , 编辑部邮箱 ,2010年03期
- 【分类号】TQ461
- 【被引频次】1
- 【下载频次】64