【摘要】 目的探索并鉴定PLC-β3与PDZ蛋白syntrophin的相互作用,为进一步研究PLC-β3在神经系统信号传导通路中的作用提供线索。方法制备GST-PLC-β3-CT融合蛋白,利用GST pull down的方法,检测PLC-β3-CT与β2-syntrophin和γ2-syntrophin的PDZ结构域的相互作用。结果构建重组质粒pGEX-PLC-β3-CT,在大肠杆菌BL21中成功表达融合蛋白后,利用GST pull down的方法证实了PLC-β3-CT可以与β2-syntrophin而不是γ2-syntrophin的PDZ结构域相互作用。结论 PLC-β3与β2-syntrophin相互作用,为研究完整的PLC-β3与β2-syntrophin的相互作用以及这种相互作用在神经系统信号网络中的功能提供了线索。更多还原

【Abstract】 Objective To identify the interaction between PLC-β3 and the PDZ domain-containing protein syntrophin,and to find the underlying roles of PLC-β3 in signaling pathway of the nervous system.Methods The carboxyl-terminal of PLC-β3(PLC-β3-CT) was fused to GST protein by gene cloning.The resultant protein was used for examining interactions of PLC-β3-CT with the PDZ-domain of β2-syntrophin and γ2-syntrophin respectively by GST pull-down assay.Results PLC-β3-CT cDNA was cloned into the expression vector pGEX-4T-1 successfully,and the GST fusion protein was overexpressed and purified in an E.coli system.GST pull-down experiments revealed that the PDZ domain of β2-syntrophin,but not γ2-syntrophin was robustly pulled down by the carboxyl-terminal of PLC-β3.Conclusion These results provide a strong evidence for further studying the intracellular association of the PLC-β3 and β2-syntrophin and for exploring their potential physiological significances in signaling pathway of the nervous system.更多还原