【摘要】 目的探讨钙调蛋白依赖性蛋白激酶(CaMKⅡ)在小鼠脑低氧预适应(HPC)发生发展中的作用。方法成年雄性BALB/c小鼠随机分为正常对照(H0)、早期(H1～H4)和延迟性(H5～H6)低氧预适应等共计7组,制备小鼠HPC模型;用Western blot并结合GelDoc凝胶成像系统,检测小鼠脑组织内CaMKⅡ磷酸化水平和蛋白表达量;用免疫组化检测小鼠脑皮层和海马CaMKⅡ磷酸化水平。结果与H0组小鼠相比,H3～H5组海马和皮层的CaMKⅡ磷酸化水平明显升高(P<0.05);而H1～H6组的皮层和海马的CaMKⅡ蛋白表达量无明显变化;H3、H6组皮层和海马p-CaMKⅡ阳性细胞数目增多和灰度增强。结论CaMKⅡ磷酸化水平的升高可能参与了小鼠脑HPC的发生发展过程。更多还原

【Abstract】 Objective To explore the role of calcium/calmodulin-dependent protein kinaseⅡ(CaMKⅡ) in the development of cerebral hypoxic preconditioning(HPC). Methods Healthy male BALB/c mice were randomly divided into 7 groups as follows:normoxic control (H0),early(H1～H4)and delayed (H5～H6) hypoxically preconditioned mice groups. SDS-PAGE,Western blot and Gel Doc imagine systems were applied to quantitatively analyze the level of CaMKⅡ phosphorylation and protein expression level in the brain of mice. Results Compared with H0 group,the phosphorylation level of CaMKⅡ increased in cortex and hippocampus of mice in H3～H5 hypoxically preconditioned groups(P<0.05). However,there was no significant changes in total CaMKⅡprotein expression in cortex and hippocampus of hypoxic preconditioned mice. Similarly,enhanced p-Thr286 CaMKⅡwas also observed in the hippocampus and cortex of mice by immunostaining following hypoxic exposures (H3 and H6). ConclusionThe increased phosphorylation of CaMKⅡ may be involved in the development of cerebral HPC in mice.更多还原