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基于质谱技术研究黑色素瘤血清特异性O-糖链

Mass spectrometry analysis of melanoma related O-glycans in serum

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【Author】 BAI Chen1,4,WU Gang1 & ZHAO Liang2,3 1 Life Science Department,Fudan University,Shanghai 200433 2 Chemistry Department,Fudan University,Shanghai 200433 3 Institutes of Biomedical Sciences,Fudan University,Shanghai 200433 4 Department of Food Science,Shanghai Business School,Shanghai 200235

【机构】复旦大学生命科学学院；上海商学院食品学院；复旦大学化学系；复旦大学生物医学研究院；

【摘要】对接种和未接种B16黑色素瘤细胞的C57小鼠进行血清O-糖链比较糖组学研究,寻找黑色素瘤血清特异性O-糖链.小鼠血清10μL,β-消除反应释放O-糖链.反应混合物经石墨化炭黑固相萃取小柱(GCC SPE)分离纯化后,用于MALDI-Qit-TOF-MS分析.通过Launchpad软件采集并输出质谱数据,MATLAB进行数据解析,找到了10个稳定出现的差异糖链质谱峰.利用串联质谱分析了其中5个主要差异糖链的结构.更多还原

【Abstract】 Glycomics comparison was carried out for screening melanoma serum biomarkers between C57 mouse with and without B16 implanted.O-glycans were released from 10 μL serum by β-elimination,purified by GCC SPE and analyzed by MALDI-QIT-TOF-MS.MS raw data was acquired and exported by Launchpad software.MATLAB was then applied for further data analysis.10 glycans were considered to be firmly changed after B16 implantation and 5 of them were picked for MS/MS for structure analysis.更多还原

【关键词】质谱；糖组学；黑色素瘤；分子标志物；
【Key words】 mass spectrometry； glycomics； melanoma； biomarkers；

【基金】上海市教育委员会科研创新项目(10YZ212);国家自然科学基金项目(30900254)资助

【文献出处】中国科学:化学 ,Scientia Sinica(Chimica) , 编辑部邮箱 ,2010年05期

【分类号】R739.5
【下载频次】321

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