【摘要】 目的了解2009年引起山东省手足口病(HFMD)的病原体型别及分布特征。方法采集HFMD临床诊断病例的粪便、咽拭子、疱疹液等标本,应用肠道通用引物和EV71、CoxA16特异性引物对其进行RT-PCR鉴定;使用RD、Hep-2细胞对部分肠道通用引物阳性的粪便标本进行病毒分离。结果共收集1604份HFMD临床诊断病例标本,实验室检测阳性标本990份,其中EV71阳性标本589份,CoxA16阳性标本29份,EV71和CoxA16同为阳性标本2份,EV71和CoxA16的检出率分别为36.72%和1.81%。从80份肠道通用引物阳性的粪便标本中分离出肠道病毒32株,其中EV71型病毒23株,CoxA16型病毒4株。结论引起山东省HFMD的病原体主要为EV71型肠道病毒,EV71感染占EV阳性标本数的59.50%。全省17个地市均有EV71感染的HFMD病例检出。更多还原

【Abstract】 Objective To reveal type and distribution of the pathogen of HFMD in Shandong Province in 2009. Methods Stool,herpes fluid,throat swab and other specimens of clinically diagnosed cases with HFMD were collected. These specimens were tested by RT-PCR assay with three primer sets:universal enterovirus,CA16-specific and EV71-specific primers; virus was isolated by RD and Hep-2 cells from the positive stool specimens of universal enterovirus primers. Results 1 604 specimens from clinically diagnosed cases with HFMD were collected. 990 cases were tested positive,including 589 cases of EV71 positive,29 cases of CoxA16 positive,2 cases of both EV71 and CoxA16 positive. The infect ratio of EV71 and CoxA16 was respectively 36.72% and 1.81%. And 32 enterovirus strains were isolated from 80 stool specimens including 23 strains of EV71 and 4 strains of CoxA16. Conclusions The HFMD in Shandong Province was mainly caused by EV71,accounted for 59.50% of the number of EV positive specimens. All cities of Shandong Province had HFMD caused by EV71.更多还原