【摘要】 目的:通过观察葶苈生脉方对充血性心力衰竭(CHF)大鼠心肌细胞凋亡的影响,探讨其治疗CHF的作用机制。方法:采用腹主动脉缩窄法复制CHF大鼠模型,随机分为假手术组、模型对照组、葶苈生脉方高剂量组、葶苈生脉方低剂量组、阳性药心宝丸对照组。观察其对心衰大鼠心肌组织形态学的影响,并运用流式细胞仪检测各组大鼠心肌细胞凋亡的情况。结果:模型组大鼠心肌纤维着色不均,纹理消失,间质轻度水肿,灶性间质细胞增生,细胞核萎缩,染色变深。流式细胞术分析可见心肌细胞凋亡率明显高于正常组(P<0.01)。葶苈生脉方高、低剂量组心肌损害均较模型组减轻,心肌纤维仅轻度颗粒样变性,间质无明显充血;心肌细胞凋亡率均较模型组降低(P<0.05或P<0.01)。结论:葶苈生脉方能抑制心衰大鼠心肌细胞的凋亡,减少心衰时心肌细胞的缺失,此可能是其治疗CHF的作用机制之一。更多还原

【Abstract】 Objective:to explore its therapeutic mechanism by observing the effect of Tingli Shengmai Fang on myocardial apoptosis of rats with congestive heart failure. Methods:CHF models,copied by abdominal aortic coarctation,were randomly divided into sham operation group,model control group,high and low dose group,Xinbao Wan control group. Then the effect was observed on myocardial morphology of CHF rats and the myocardial apoptosis of each group detected with flow-type cytometry. Results:In model group,myocardial fiber with uneven coloring could be seen,with texture disappeared,interstitial edema mildened,focal interstitial cell proliferated,nuclear shrinked and chromatin deepened. Flow cytometry analysis showed that the apoptosis was significantly higher than that of normal group (P<0.01). In both high and low group,compared with model group,the myocardial damage reduced,with slightly granular degeneration and no obvious interstitial hyperemia,and apoptosis was lower than that of model group (P<0.05 or P<0.01). Conclusion:Tingli Shengmai Fang can inhibit the myocardial apoptosis of CHF rats and reduce the loss of myocardial cells during heart failure,which may be one of the mechanisms of treating CHF.更多还原