【摘要】 通过携带Mini-Tn10转座子的温度敏感性质粒载体pIC333构建了苏云金芽胞杆菌YBT-1518菌株的插入突变体库。从突变体库中筛选得到1株芽胞不能在萌发剂L-丙氨酸诱导下萌发的突变株(命名为MA1),进一步的试验表明该突变株菌株的芽胞在其他氨基酸L-苯丙氨酸、L-缬氨酸和L-亮氨酸以及糖类萌发剂葡萄糖、果糖和蔗糖的诱导下也都不能萌发,表明MA1很可能属于新型的芽胞萌发缺陷突变株。更多还原

【Abstract】 A insertional mutant library of Bacillus thuringiensis strain YBT-1518 was constructed with a thermo-sensitive plasmid pIC333 carrying Mini-Tn10.A mutant(named MA1)defective in germination response to L-alanine was isolated from the library by detecting with phase-contract microscopy and D600 nm.Further studies indicate that MA1 was defective in germination response to other amino acid(such as L-phenylalanine,L-valine and L-leucine)and sugars(such as glucose,sucrose and fructose),different from other reported mutants.Results indicate that MA1 is a new type defective in spore germination.更多还原