【摘要】 以草鱼、鮰鱼、鳝鱼为原料,从3种淡水鱼肌肉中提取酸性磷酸酶(ACP),对粗酶液的酶学特性进行了研究。结果表明:草鱼、鮰鱼和鳝鱼肌肉中ACP适宜反应pH值分别为5.0、5.8和5.6,适宜反应温度分别为60、37、43℃;草鱼、鳝鱼肌肉中ACP在pH值为5～9条件下较稳定,鮰鱼肌肉ACP在pH值为5～8条件下较稳定;Na+、K+、Mg2+对酶活性影响不大,而Zn2+对ACP有激活作用,Cu2+、Ca2+、Hg2+对酶活性有抑制作用;半胱氨酸、磷酸钠、焦磷酸钠对鱼肉ACP酶活性有较好的抑制效果。更多还原

【Abstract】 The acid phosphatase(ACP) from grass carp,channel catfish and mud eel muscles were extracted and partial properties of the crude enzyme were investigated.The results showed that the optimal pH values of ACP of grass carp,channel catfish and mud eel muscle were 5.0,5.8 and 5.6,respectively.The optimal temperatures were 60 ℃,37 ℃ and 43 ℃,respectively.ACP of grass carp and mud eel muscle were stable in the pH range of 5-9,while ACP of channel catfish was in the pH range of 5-8.Na +,K+,Mg2 + have no effect on the enzymes,whereas ACP activity was stimulated by Zn2 + and inhibited by Cu2 +,Ca2 +,Hg2 +.ACP activity was also inhibited by cystine,sodium phosphate,sodium pyrophosphate.更多还原