【摘要】 应用NCBI、Expasy等在线生物信息学网站与生物信息学软件对蓝细菌各菌株的PipX进行多序列同源对比分析,获得保守序列,预测了二级结构与结构域,分析了pipX的基因簇等。分别构建了蓝细菌的16S rDNA进化树以及基于PipX的分子进化树,同时也对与PipX相互作用的NtcA和PⅡ构建分子进化树进行了比较分析,了解它们之间起源与进化的关系。由此推测PipX可能是蓝细菌氮代谢途径中除NtcA、PⅡ外的另一个关键调控蛋白,有着重要的生理功能。更多还原

【Abstract】 The multi-sequence homologous alignment of PipX in cyanobacteria and the conserved sequences were analyzed by bioinformatics softwares and websites such as NCBI,Expasy and so on.The secondary structure and structural domain of PipX was predicted and pipX gene clusters were dissected.The phylogenetic tree of 16S rDNA in cyanobacteria was constructed.In addition,the phylogenetic relationships among PipX,NtcA and PⅡ were studied by constructing phylogenetic trees of these genes.The results indicated that PipX is an important regulation protein in the nitrogen metabolism of cyanobacteria in addition to NtcA and PⅡ,and might have important physiological functions.更多还原