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豌豆根尖铝毒的敏感浓度探讨

Sensitive Concentration of Al3+ to Root Tip of Pea(Pisum sativum)

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【作者】 何丽烂喻敏王惠珍刘家友刘莉莉

【Author】 HE Li-lan YU Min WANG Hui-zhen LIU Jia-you LIU Li-li Department of Horticulture,Foshan University,Guangdong Province,Foshan 528000,China

【机构】 广东省佛山科学技术学院园艺系

【摘要】 以不同浓度Al3+处理豌豆幼根根尖,24 h后通过测定幼根的相对伸长率、不同根段的胼胝质含量、POD活性同时结合苏木精染色,探讨不同浓度Al3+对豌豆根尖生长的影响。结果表明:幼根伸长明显受到抑制,苏木精染色的颜色也随Al3+浓度上升而加深;不同根段的胼胝质含量明显增加,当Al3+浓度大于50μmol/L后,差异达到显著;幼根的POD活性显著降低。说明Al3+抑制豌豆幼根的生长,其敏感浓度为50μmol/L。

【Abstract】 Influences of different concentration of Al3+ on the roots of pea(Pisum sativum) were studied by determining root elongnation,callose production,activity of peroxide(POD) and hematoxylin stain of root apices after treated by Al3+ for 24 h.The results showed that root elongation was obviously inhibited and activity of POD decreased with the increase of Al3+ concentration.Callose content increased with the increase of Al3+ concentration and significantly increased at Al3+ concentration over 50 μmol/L accompanied with heavy hematoxylin stain.These results indicated that Al3+ inhibited root growth and the sensitive concentration of Al3+ to root tip of pea is 50 μmol/L.

【关键词】 豌豆根尖Al3+胼胝质过氧化物酶
【Key words】 pea(Pisum sativum)root tipAl3+calloseperoxide
【基金】 国家自然科学基金项目(30471035);广东省自然科学基金项目(06029443)资助
  • 【文献出处】 华中农业大学学报 ,Journal of Huazhong Agricultural University , 编辑部邮箱 ,2010年01期
  • 【分类号】S436.43;X173
  • 【被引频次】14
  • 【下载频次】278
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