【摘要】 分别采用低浓度玉米小斑病菌Hel minthospolium maydis T小种毒素(HMT毒素)和玉米小斑病菌T小种培养滤液中的提取蛋白及菌丝细胞壁的提取液,预处理不同玉米品种3叶期叶片后再接种高浓度HMT毒素,在无菌条件下培养5 d后测量叶片病斑大小,并测定玉米叶片内苯丙氨酸解氨酶(PAL)活性变化。结果表明:只有纯化的低浓度HMT毒素能够作为激发子诱导玉米获得系统性广谱抗性,但不同玉米品种所需的最适合浓度有差异;TC103以质量浓度0.025μg/mL毒素预处理效果最好,PAL活性最高;TB37和TMo17 2个自交系,以质量浓度分别为0.025μg/mL和0.050μg/mL的毒素预处理效果最好。更多还原

【Abstract】 The maize leaves were pretreated respectively by low concentraion Helminthospolium maydis T-toxin(HMT-T),protein and cell-wall,which were extracted from cultivating filtrate at the stage of three-leaf,and then these leaves were treated by high concentration HMT-T and cultivated 5 d.The dimension of spots in infected leaves was calculated and phenylalanine ammonia lyase(PAL) activity determined.The result showed that only the HMT-T could act as elicitor in the cultivating filtrate.The suitable concentration of HMT-T was different for various varieties.As to TC103 the suitable concentration of toxin was 0.025 μg/mL and the PAL activity of maize leaves reached its maximum degree in this case.The right concentration of TB37 and TMo17 were 0.025 μg/mL and 0.050 μg/mL respectively.更多还原