【摘要】 目的检测结肠癌中VEGF-D及其受体VEGFR-3、LYVE-1表达,探讨其在结肠癌淋巴管新生中的价值。方法选择90例结肠癌术后新鲜标本作为实验组,90例正常结肠黏膜新鲜组织作为对照组,应用流式细胞仪检测VEGF-D、VEGFR-3及LYVE-1的表达,分析其在肿瘤中表达的意义及在不同临床病理特征之间的关系。结果 VEGF-D、VEGFR-3、LYVE-1在结肠癌中的表达分别为1.41±0.19、1.39±0.28、1.34±0.23,在正常结肠黏膜分别为1.00±0.21、1.00±0.23、1.00±0.26,VEGF-D、VEGFR-3及LYVE-1在结肠癌中的表达均明显高于正常结肠黏膜组织(P<0.05)。VEGF-D、VEGFR-3、LYVE-1在43例(肿瘤大小<3 cm)及47例(肿瘤大小≥3 cm)的结肠癌中的表达分别为1.24±0.22、1.31±0.25、1.27±0.19及1.57±0.131、1.46±0.22、1.40±0.19;在50例(浸润深度未及浆膜)及40例(浸润深度已达浆膜及以外)的结肠癌中的表达分别为1.33±0.20、1.31±0.21、1.24±0.22及1.51±0.18、1.49±0.23、1.47±0.18;在44例(Dukes分期为A+B)及46例(Dukes分期为C+D)的结肠癌中的表达分别为1.32±0.22、1.31±0.22、1.25±0.20及1.50±0.14、1.47±0.24、1.43±0.24,P值均<0.05,VEGF-D、VEGFR-3及LYVE-1在结肠癌中的表达与结肠癌的肿瘤大小、浸润深度、Dukes分期及淋巴结转移密切相关,结肠癌中VEGF-D、VEGFR-3与LYVE-1的表达存在正相关(r=0.48,0.40,0.44,P<0.05)。结论 VEGF-D、VEGFR-3及LYVE-1可能与结肠癌的发生发展有关,VEGF-D与VEGFR-3参与LYVE-1阳性的淋巴管生成,VEGF-D、VEGFR-3及LYVE-1可能与结肠癌患者的预后有一定关系。更多还原

【Abstract】 Objective To detect the expressions of VEGF-D and VEGFR-3,LYVE-1 in colorectal carcinoma,and investigate its relationship in pathogenesis,development and lymphangiogenesis in neoplasm.Methods The study included 90 cases of colorectal carcinoma as experimental group and 90 cases of normal tissues as control group.We analyzed their expressions of VEGF-D,VEGFR-3 and LYVE-1 in different clinical and pathological character by using the Flow Cytometry. Results The expressions of VEGF-D,VEGFR-3 and LYVE-1 were obviously higher in the colorectal carcinoma(1.41±0.19,1.39±0.28,1.34±0.23) than in the normal mucosa(1.00±0.21,1.00±0.23,1.00±0.26).The expressions of VEGF-D,VEGFR-3 and LYVE-1 were obviously higher in more 3 cm group(1.57±0.131,1.46±0.22,1.40±0.19) than in the less 3cm group(1.24±0.22,1.31±0.25,1.27±0.19).The expressions of VEGF-D,VEGFR-3 and LYVE-1 were obviously higher in deeper invasion group(1.51±0.18,1.49±0.23,1.47±0.18)than in the shallower invasion group(1.33±0.20,1.31±0.21,1.24±0.22).The expressions of VEGF-D,VEGFR-3 and LYVE-1 were obviously higher in C and D stage group(1.50±0.14,1.47±0.24,1.43±0.24) than in the A and B stage group(1.32±0.22,1.31±0.22,1.25±0.20).The expressions of VEGF-D,VEGFR-3 and LYVE-1 were correlated with tumor volume,invasion,Dukes stage and lymph metastasis in colorectal carcinoma.Positive correlation between VEGF-D and VEGFR-3(r=0.48,P=0.0004)),VEGF-D and LYVE-1(r=0.40,P=0.0154),VEGFR-3 and LYVE-1(r=0.44,P=0.0046) were found in colorectal carcinoma. Conclusions VEGF-D,VEGFR-3 and LYVE-1 are correlated with the occurrence and development of colorectal carcinoma.VEGF-D and VEGFR-3 promote the lymphangiogenesis which expressed LYVE-1.VEGF-D,VEGFR-3 and LYVE-1 may be correlated with prognosis in colorectal carcinoma.更多还原