【摘要】 应用流式细胞仪技术,通过检测血细胞的吞噬活力、活性氧产物和死亡率,研究了Cd2+和苯并芘(Bap)胁迫7d对文蛤血细胞功能的影响。结果表明,10μg/L和100μg/LCd2+胁迫会显著降低血细胞的吞噬活力,增加血细胞的死亡率,100μg/LCd2+会显著增加血细胞活性氧产物。5μg/L和50μg/LBap胁迫会显著降低文蛤血细胞的吞噬活力,增加血细胞的死亡率,50μg/LBap会显著增加血细胞活性氧产物。可见,Cd2+和苯并芘胁迫会显著降低文蛤血细胞的功能,导致文蛤免疫力下降,增大文蛤被病害感染的机率。更多还原

【Abstract】 The effects of cadmium and benzo[α]pyrene(Bap) exposure on the immune function of Meretrix meretrix hemocytes were examined by flow cytometery.The results showed that 10 and 100 μg/L Cd2+ significantly suppressed phagocytosis of hemocytes and increased the mortality of hemocytes,while 100 μg/L Cd2+ stimulated reactive oxygen species(ROS) in hemocytes.Bap(5 and 50 μg/L) inhibited phagocytosis and increased the mortality of hemocytes,while 50 μg/L Bap could enhance ROS.The results suggested that cadmium and benzo[α]pyrene exposure significantly inhibited the immune function of hemocytes,which may lead to low immunity and increased infection of M.meretrix.更多还原