【摘要】 采用二因素三水平设计试验,用添加不同水平配比的VE和Se(mg/kg)的饵料G0(0,0)、G1(200,0.2)、G2(200,0.4)、G3(200,0.8)、G4(400,0.2)、G5(400,0.4)、G6(400,0.8)、G7(800,0.2)、G8(800,0.4)、G9(800,0.8)投喂凡纳滨对虾,研究VE和Se对体长3cm左右凡纳滨对虾体液抗氧化系统的影响,试验进行4周。结果表明,第2周时,VE、VE和Se对O2?、酚氧化酶(PO)、血清总抗氧化能力(T-AOC)有显著影响(P<0.05);Se对PO、血清和肝胰腺中T-AOC以及肝胰腺中GPx有显著影响(P<0.05)。第4周时,VE和Se协同作用对4种抗氧化指标都有显著影响(P<0.05)。第2、4周时,抗氧化酶活力比第0周显著升高。对O2?的研究发现,在第2和第4周时,G8组血细胞O2?最多。第2周时,血清PO在G5、G9组活力最高(P<0.05),两组间差异不显著(P>0.05);血清和肝胰腺中T-AOC在G2、G3组最高(P<0.05);对虾肝胰腺中GPx酶活力在G3和G5组达到最高(P<0.05),两组间差异不显著(P>0.05)。第4周时,G7组PO和GPx活力最高,G9组T-AOC活力达到最高(P<0.05)。研究结果提示:添加适量的VE和Se能显著提高对虾抗氧化能力,VE和Se对抗氧化系统具有随时间变化的动态调节作用,VE和Se之间存在交互作用。当在基础饵料中分别添加VE和Se在400mg/kg、0.4mg/kg时,凡纳滨对虾机体抗氧化能力整体达到平衡,能有效抵制氧自由基的损伤。更多还原

【Abstract】 Litopendeus vannamei (PL 3cm) samples were divided randomly into 10 groups in triplication, each con- tained 30 individuals. A matrix of three-level by two-factor was designed. The shrimp were fed with vitamin E and sele- nium for each group, respectively as: G0 (0, 0), G1 (200, 0.2), G2 (200, 0.4), G3 (200, 0.8), G4 (400, 0.2), G5 (400, 0.4), G6 (400, 0.8), G7 (800, 0.2), G8 (800, 0.4), and G9 (800, 0.8mg/kg). The experiment lasted for four weeks during which the effects of different levels of vitamin E and selenium on O2? in haemocytes, Phenoloxidase (PO) activity and to- tal-antioxidative activity (T-AOC) in serum and hepatopancreas, and glutathione peroxidase (GPx) activity in hepatopan- creas, were studied. The antioxidant activity was checked at 0, 2, and 4 weeks. The result shows that vitamin E and sele- nium could enhance the antioxidant ability dynamically. The optimal point of adding scheme was at 400mg/kg vitamin E and 0.4mg/kg selenium into the basal diets, at which the overall antioxidant system of the shrimp would reach the balance for fighting against the damage by oxygen radical.更多还原