【摘要】 在水温(15±1)℃条件下,给大菱鲆以100 mg/kg的剂量灌服磺胺二甲嘧啶后,用HPLC法测得96h内大菱鲆肌肉、血浆、肝脏中磺胺二甲嘧啶的浓度。用DAS 2.0软件分析后表明,磺胺二甲嘧啶在大菱鲆肌肉、血浆和肝脏中药代动力学模型均为一房室开放模型,肌肉、血浆和肝脏中的消除相半衰期T1/2和吸收相半衰期T1/2ka分别为27.413h、8.8h、5.715h和1.526h、0.919h、1.358h;出现达峰时间Tmax为8h、4h、6h;最高药物浓度Cmax为31.486μg/mL、40.256μg/mL、26.475μg/mL;总体消除率CL/F为0.069L/h.kg、0.142L/h.kg、0.312L/h.kg;药物浓度-时间曲线下面积AUC为1178.428 mg/L.h、702.739 mg/L.h、320.015 mg/L.h;建议在水温15℃下使用磺胺二甲嘧啶进行大菱鲆疾病的预防和治疗时,至少停药36d后方可上市销售。更多还原

【Abstract】 100mg/kg dose of sulfamethazine was given to turbot by oral administration at 15±1℃.The contents of sulfamethazine within 96h were determined with High Performance Liquid Chromatography.The concentrations of sulfamethazine in muscle,blood and liver of turbot were best described as a one-compartment open model with lagtime by DAS.2.0.The main parameters were as follows: T1/2 and T1/2ka were 27.413h,8.8h,5.715h,and 1.526h,0.919h,1.358h;Tmax were 8h,4h,6h;Cmax were 31.486μg/mL,40.256μg/mL,26.475μg/mL;CL/F were 0.069L/h·kg,0.142 L/h·kg,and 0.312 L/h·kg;AUC were 1178.428mg/L·h,702.739 mg/L·h,and 320.015 mg/L·h.It was suggested that turbot came into market at least 50d after ceasing utilization of the drug when SM2 was used for disease prevention and treatment.更多还原