【摘要】 目的通过建立大鼠脑皮层神经元缺氧复氧模型,探讨还原型辅酶Ⅰ(reduced nicotinamide adenine dinu-cleotide,NADH)对缺氧复氧诱发神经元凋亡的保护作用。方法原代培养新生大鼠脑皮层神经元,随机分成正常对照组,缺氧复氧组和NADH组。用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL法)检测细胞凋亡,计算凋亡率,检验各试验组细胞凋亡率的差异。用透射电镜观察凋亡细胞超微结构。结果正常对照组各阶段细胞凋亡率间差别无统计学意义(P>0.05)。缺氧复氧组和NADH组凋亡率均较正常对照组调亡率大,差异有统计学意义(P<0.01),缺氧复氧组较NADH组凋亡率大,差异有统计学意义(P<0.01)。在复氧12 h内给予NADH,对于神经元凋亡抑制作用优于其余复氧时间点。结论缺氧复氧可诱发体外培养神经元发生凋亡,NADH对此有明显抑制作用。在复氧12 h内给予NADH对凋亡的抑制作用较明显。更多还原

【Abstract】 Objective To observe the protective role of NADH on apoptosis caused by the hypoxia/reoxygenation in primary cultured cortical neurons in rat.Methods Primary neurons from neonate rat cortex were cultured.The neurons in good condition were divided randomly into 3 groups:control group,hypoxia/reoxygenation group and NADH group.The ultrastructure of apoptosis cells were observed by using transmission electron microscope.Apoptosis was detected by TUNEL assay.The differences of apoptosis rate between those groups were calculated and tested.Results The apoptosis rate of the normal control group at various stages had no significant difference(P>0.05).Compared with the control group,the apoptosis rate of hypoxia-oxygen group and NADH group were higher(P<0.01).The apoptosis rate of hypoxia-oxygen group was higher than that of NADH group(P<0.05).NADH worked better at reoxygenat 12 h than the other time.Conclusion NADH has a significant effect on protecting neurons from apoptosis induced by hypoxia/reoxygenation.NADH works better at reoxygenat 12 h than the other time.更多还原