【摘要】 制备了磁性四氧化三铁纳米粒子,采用碳纳米管、Nafion等功能性材料构建了酪氨酸酶(Tyr)生物传感器,并将其应用于农药的检测.实验表明,四氧化三铁纳米粒子具有良好的生物兼容性,碳纳米管能够有效地促进电子传递,修饰了四氧化三铁纳米粒子等功能性材料的酶传感器,响应速度快,检测灵敏度高,稳定性好;固定在传感器上的酪氨酸酶有良好的酶动力学响应,其表观米氏常数为61.5μmol/L.利用农药对酪氨酸酶的抑制作用,以苯酚为底物,对农药呋喃丹进行了检测,检测限达到2.0×10-9mol/L.更多还原

【Abstract】 A Tyr/Glu/Fe3O4/Nafion/CNT multilayer modified electrode was employed for the detection of carbofuran in samples. The configuration of the nanostructure on the electrode provides a favorable environment for the Tyr electrocatalytic characterization and fast electron transfer. The surface of the sensor was characterized by TEM methods. A set of experimental conditions were also optimized for the detection of the pesticide. The developed biosensor showed high sensitivity, stability and reproducibility, which had a low detection limit of 2.0×10-9 mol/L for carbofuran.更多还原