【摘要】 目的检测临床再治疗根管内的粪肠球菌,分析粪肠球菌检出率与临床症状及体征之间的关系。方法临床收集需根管再治疗的患牙108颗,记录症状和体征,根管内采集细菌样本,提取细菌基因组DNA,用聚合酶链反应定性检测粪肠球菌。结果根管内粪肠球菌的检出率为47.2%。在有症状病例、有体征病例、既有症状又有体征病例中,根管内粪肠球菌的检出率分别为52.6%、57.9%、62.5%,其中,有体征病例与无体征病例粪肠球菌的检出率差异有统计学意义(P<0.05),既有症状又有体征病例与无体征病例组粪肠球菌的检出率差异有统计学意义(P<0.05);在有症状的病例组中,有咬合痛的病例粪肠球菌的检出率为66.7%,与无咬合痛病例组相比差异有统计学意义(P<0.05)。结论再治疗根管内粪肠球菌的存在与临床症状或体征密切相关。更多还原

【Abstract】 Objective To detect the Enterococcus faecalis(E.faecalis) in post-treatment endodontic disease,and to analyze the relationship between the occurrence of E.faecalis and clinical symptom.Methods 108 teeth which need root canal retreatment were collected,and the clinical symptoms and physical signs were recorded.Bacterium samples from root canal were taken,and genome DNA from bacterial samples were extracted.The occurrence of E.faecalis by means of the polymerase chain reaction was investigated.Results The detection rate of E.faecalis in cases of root canal retreatment was 47.2%,while in cases with symptoms or signs,or cases with both symptoms and signs,the root canal E.faecalis detection rates were 52.6%,57.9%,62.5%.The detection rates of E.faecalis between cases with clinical symptom and without clinical symptom demonstrated statistical significance(P<0.05).The detection rates between cases with both clinical symptom and manifestly aneretic root and cases without clinical symptom and manifestly aneretic root had statistical significance(P<0.05).In the group of clinical symptom,the detection rate of E.faecalis in cases with biting pain was 66.7%,clearly higher than those without biting pain(P<0.05).Conclusion The occurrence of E.faecalis in cases of root canal retreatment correlates with clinical symptoms.更多还原