【摘要】 目的探讨CMV感染对冠心病患者血清IL-10的影响。方法冠心病患者106例和冠状动脉造影证实的冠状动脉光滑者82例作为对照,ELISA法检测特异性CMV IgG,双单抗夹心法定量测定血清IL-10水平。据研究个体的IL-10中位数水平(6.84 pg/ml)进行高、低IL-10分组,行单因素和多因素Logistic回归分析高水平IL-10的影响因素。结果冠心病组与对照组相比,CMVIgG阳性率差异不显著(38.9%vs.37.6%,P>0.05);冠心病组IL-10水平显著高于对照组,7.3(3.0-12.5)pg/ml vs.5.9(3.2-10.4)pg/ml(P=0.043)。高IL-10患者的CMV IgG阳性率显著高于低IL-10患者(46.8%vs.30.4%,P<0.001);在冠心病组,高IL-10组中的CMVIgG阳性率显著高于低IL-10组(48.1%vs.28.6%,P<0.001),而在冠状动脉光滑患者的高、低IL-10组中,未见CMV IgG阳性率的显著差异(40.4%vs.35.6%,P=0.588);IL-10升高相关因素的多元Logistic回归分析显示,在冠心病组,CMV IgG阳性与高IL-10独立相关,调整的OR值为2.3(1.6～3.4,P<0.001),而在对照组则为1.1(0.5～2.5,P=0.83)。调整变量包括年龄、性别、吸烟、高血压、血脂紊乱、糖尿病及较少体力活动。结论CMV感染与冠心病患者血清IL-10水平升高独立相关,在冠心病患者,CMV感染的免疫应答可能是一个影响细胞因子网络的复杂反应。更多还原

【Abstract】 Objective Investigated the impact of CMV infection on serum IL-10 levels in patients with CAD.Methods Specific CMV IgG and serum levels of IL-10 were measured with ELISA in patients with CAD(n = 106) and smooth coronary artery controls documented angiographically(n = 82).Subjects were dichotomized according to calculated median level of IL-10(6.84 pg/ml) in different groups or subgroups. Univariate and multivariate analyses were performed to analyse the contributors of high IL-10.Results The seropositivity of CMV IgG was not significantly different between CAD and control groups (38.9%vs.37.6%,P>0.05).IL-10 level in the patients with CAD was higher than that in the controls [7.3(3.0-12.5) pg/ml vs.5.9(3.2-10.4) pg/ml,P = 0.043].The seropositivity of CMV IgG was more frequently found in the high IL-10 group than the low IL-10 group(46.8%vs.30.4%,P<0.001). The prevalence of CMV infection was significantly higher in the high IL-10 group than the low IL-10 group among the patients with CAD(48.1%vs.28.6%,P<0.001),but not among the controls(40.4%vs. 35.6%,P = 0.588).On multiple logistic regression analysis,the adjusted odds ratio of high IL-10 associated with CMV infection was 2.3(1.6～3.4,P<0.001) in the patients with CAD,and 1.1(0.5～2.5, P = 0.83) in the controls.Conclusion We found a significant association of CMV infection with elevated IL- 10 in patients with CAD,but not in those with smooth coronary artery.Therefore,we propose that changes in the immune response to CMV are a compounding factor in CAD,更多还原