【摘要】 利用均匀设计法,优化一株黏质沙雷氏菌(Serratia marcescens)产几丁质酶培养基的组分;同时,利用正交设计法优化其摇瓶发酵产酶的条件.研究结果表明,最佳培养基组分(质量分数):0.504%胶体几丁质,1.178%酵母粉,0.025%MgSO4.7H2O,0.095%K2HPO4,0.001%FeSO4.7H2O,0.005%山梨醇,0.030%KH2PO4;最佳摇瓶发酵工艺条件:培养时间为48 h,发酵温度为30℃,初始pH值为9.0,装液量为30 mL,接种量为1%.在此优化条件下,酶活力可达9.39μkat.L-1,比未优化的产酶条件下酶活力提高了81.6%.更多还原

【Abstract】 Chitinase-producing Serratia marcescens culture medium components and culture conditions of enzyme production by shake flask fermentation were optimized with uniform-design and orthogonal-design respectively.The results showed that the optimal component concentrations(m/m) were colloid chitin 0.504%,yeast extract 1.178%,MgSO4·7H2O 0.025%,K2HPO4 0.095%,FeSO4·7H2O 0.001%,sorbitol 0.005%,KH2PO4 0.030%.The optimal culture conditions were incubation time 48 h,temperature 30 ℃,initial pH 9.0,loadage 30 mL per 250 mL flask,inoculation quantity 1%.The activity of chitinase reached 9.39 μkat·L-1 which was increased by 81.6% than that of the unoptimized conditions..更多还原