【摘要】 目的:研究缺氧诱导因子-1α(HIF-1α)及脆性组氨酸三联体基因(FHIT)蛋白在食管鳞癌组织中的表达及其意义。方法:应用免疫组织化SP法检测48例食管鳞癌及癌旁组织中HIF-1α及FHIT蛋白的表达,分析其与食管鳞癌病理特征的关系。结果:食管鳞癌组织中HIF-1α的总阳性表达率明显高于癌旁组织(P<0.05),而FHIT蛋白的总阳性表达率明显低于癌旁组织(P<0.05);在食管鳞癌组织中HIF-1α的表达与FHIT蛋白的表达呈负相关(P=0.006,r=-0.392);HIF-1α及FHIT蛋白在食管鳞癌组织中的表达与浸润深度(pTNM分期)及淋巴结转移有明显关系(P<0.05),而HIF-1α表达还与癌组织分化程度相关(P<0.05),但均与年龄、性别等因素无关(P>0.05)。结论:HIF-1α及FHIT蛋白的异常表达在食管鳞癌的发生、发展中起重要作用,在一定程度上反映了食管鳞癌侵袭性强弱,且HIF-1α高表达与FHIT蛋白低表达存在相关性,FHIT的检测有利于食管鳞癌患者的早期诊断。更多还原

【Abstract】 Objective: To study the expressions of HIF-1α and FHIT protein in esophageal squamous carcinoma tissue.Methods: By using S-P method of immunohistochemical technique,the expression of HIF-1α and FHIT protein in 48 specimens of esophageal squamous carcinoma tissue and its corresponding adjacent tissu were detected,and their relationship with clinicopathological characteristics were analyzed.Results: The positive expression intensity of HIF-1α in esophageal squamous carcinoma tissue was remarkably stronger than that in its corresponding adjacent tissue(P<0.05),while the expression intensity of FHIT protein was weaker in esophageal squamous carcinoma tissue(P<0.05).There was negative correlation between the positive expression rate of HIF-1α protein and that of FHIT protein in esophageal squamous carcinoma tissue(P<0.01,r=-0.392).The expression of HIF-1α and FHIT protein in esophageal squamous carcinoma tissue were correlated with the invasion depth(pTNM tumor stage) and lymph node metastasis(P<0.05),and the expression of HIF-1α was correlated with the differentiation of tumors(P<0.05),but had no correlation with age and gender(P>0.05).Conclusions: The abnormal expressions of HIF-1α and FHIT protein in esophageal squamous carcinoma tissue suggest that they probably play some roles in the origin and development of esophageal squamous carcinoma,and it reflects the differentiation and invasive potential of esophageal squamous carcinom tissues to same degrees.There is significant correlation between the expression of HIF-1α and FHIT.The determination of FHIT may help in early diagnosis of esophageal squamous cell carcinoma patients.更多还原