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维生素E抑制3T3-L1前脂肪细胞的分化
Inhibitory effect of vitamin E on differentiation of 3T3-L1 preadipocyte
【摘要】 目的:研究维生素E对3T3-L1前脂肪细胞增殖和分化的影响。方法:利用MTT研究维生素E对3T3-L1前脂肪细胞增殖的影响,利用油红O染色法测定维生素E对3T3-L1前脂肪细胞分化的影响。结果:维生素E对3T3-L1前脂肪细胞增殖无影响,10~100μmol/L维生素E能抑制3T3-L1前脂肪细胞的分化。结论:维生素E对前脂肪细胞分化具有抑制作用。
【Abstract】 Objective:To study the effects of vitamin E on proliferation and differentiation of 3T3-L1 preadipocyte.Methods:The effect of vitamin E on the proliferation of 3T3-L1 was determined by MTT.Oil red O staining was used to determine the differentiation rate and level of 3T3-L1 preadipocytes.Results:Vitamin E inhibited the differentiation of preadipocytes at 10 μmol/L and 100 μmol/L.Conclusions:Vitamin E could inhibit the differentiation of 3T3-L1 preadipocyte.
【关键词】 维生素E;
碘;
3T3-L1;
增殖;
分化;
肥胖;
MTT;
油红O染色法;
【Key words】 Vitamin E; Iodine; 3T3-L1; Proliferation; Differentiation; Obesity; MTT; Oil red staining;
【Key words】 Vitamin E; Iodine; 3T3-L1; Proliferation; Differentiation; Obesity; MTT; Oil red staining;
【基金】 海南省自然科学基金资助项目(808164)~~
- 【文献出处】 海南医学院学报 ,Journal of Hainan Medical University , 编辑部邮箱 ,2010年09期
- 【分类号】Q566
- 【被引频次】5
- 【下载频次】253