【摘要】 目的:观察甲基强的松龙(mPSL)对MC3T3-E1成骨细胞L-钙通道电流(L-type voltage-sensi-tive calciumchannels current,L-VSCCsC)的影响。方法:设A组(正常对照组)、B组(10-6mol/L)、C组(10-5mol/L)和D组(10-4mol/L),各浓度的mPSL作用24h后,膜片钳技术记录每组细胞的L-VSCCsC(nA)。结果:正常对照组峰值电流为(-0.2284±0.0209,nA);10-6mol/L组为(-0.1991±0.0158,nA)下降12.8%;10-5mol/L和10-4mol/L组分别为(-0.1839±0.0179,nA)、(-0.1610±0.0033,nA)分别下降19.5%和29.5%,与正常对照组比较有显著性差异(P<0.01)。结论:mPSL剂量依赖性抑制成骨细胞L-VSCCsC。更多还原

【Abstract】 Objective:To observe the influence of methylprednisolone on the Ca2+ channel currents in osteoblasts.Methods:All were divided into four groups,A(control),B(10-6mol/L)、C(10-5mol/L)and D(10-4mol/L).The whole cell patch clamp technique was used in every MC3T3-E1 osteoblast of each group to test L-type voltage-sensitive calcium channels current(L-VSCCsC).Results:The mean peak current of control group was(-0.2284±0.0209,nA),B group was(-0.1991±0.0158,nA)with 12.8% decrease;C and D groups were(-0.1839±0.0179,nA)and(-0.1610±0.0033,nA)with 19.5% and 29.5% decrease,respectively.There was significant difference as compared with control(P<0.01).Conclusion:Methylprednisolone can inhibit the currents of L-calcium channels dose-dependently.更多还原