【摘要】 目的:利用基因芯片筛选与甲状腺乳头状癌发生、发展和与恶性度相关的基因。方法:分别选取高、低分化甲状腺乳头状癌组织标本,抽提、纯化mRNA,同法再纯化同患者的正常甲状腺组织的mR-NA;用Cy5荧光染料对甲状腺乳头状癌组织mRNA作探针标记,以Cy3荧光染料对正常黏膜组织的mRNA作探针标记,一同和可检测4096个基因片段的BiostarH-40s型基因芯片进行杂交得到的双色荧光标记芯片,将之分别扫描入计算机,进行结果分析。结果:高、低分化2种芯片上均有大量基因片段出现了的高/低表达的情况,高分化芯片上异常表达片段数量为低表达351条,高表达417条,低分化芯片上异常表达者为低表达410条,高表达415条。在2种芯片中均有相同表达差异趋势的基因片段中,共计有90条基因片段出现低表达,其中随恶性度增高而降低的基因有19条;有78条片段呈现高表达状态,其中随恶性度增高而表达增加的有25条。表达异常的片段功能较分散;有大量的不明功能的片段发现。结论:甲状腺乳头状癌的发生、发展不能用单一的基因变异解释,而是由多基因共同作用的结果,并且变异具有明显的个体性差异;甲状腺乳头状癌的恶性度与多个基因片段异常表达相关。很多新发现的片段的功能不明了,对于确定甲状腺乳头状癌的发生发展内在机制仍有待研究。更多还原

【Abstract】 Objective:To screen genes that were correlative with carcinogenesis,development and malignance of papillary thyroid carcinoma using cDNA microarray.Method:Two pieces of papillary thyroid carcinoma tissue with different malignance degree and samples from normal thyroid tissue were collected for mRNA extraction and purification.Marked the purified mRNA from carcinoma tissue with Cy5 deoxycytidine triphosphate for probes labeling and mRNA from normal tissue with Cy3-dCTP separately,mix these two kinds of mRNA probes together and then hybridize to the cDNA microarray.If Cy3 signal is stronger,the spot on the microarray would be green,means low-expressed gene.While stronger Cy5 signal will lead to a red spot,which means over-expressed gene.Experiment gets 2 pieces of double-color cDNA microarrays and then they were scanned into computer and analyzed.Result:Differences were found between carcinoma tissue and normal thyoid tissue in their gene fragments’ expression.In the well-differentiated chip,there are 351 low-expressed and 417 over-expressed gene fragments presented.To the poorly differentiated chip,the correspondence number was 410 and 415.90 low-expressed and 78 over-expressed genes fragments were individually presented in both chip segments with similar expression trend,of which,expression of 25 seemed correlated to malignant degree.Large quantity of genes with unknown functions were found.Conclusion:The carcinogenesis,development and malignance degree of papillary thyroid carcinoma is resulted from different kinds of abnormality of polygenes not a single gene.Since functions of many gene fragments has been unknown to us yet,further study still needs to be done.更多还原