【摘要】 目的:探讨脑灵汤对阿尔茨海默病(AD)模型大鼠海马CA3区APP蛋白表达的影响,并揭示AD发病的部分机制和脑灵汤可能的作用机制。方法:选取40只SPF清洁级SD大鼠,随机分为正常对照组、假手术组、AD组、脑灵汤组和脑复康组,每组8只,喂养1周后,采用Aβ1-42注射大鼠海马法制成大鼠模型,再喂养28 d,治疗后用Morris水迷宫实验检测大鼠学习记忆行为能力,HE染色检测大鼠海马组织形态学变化,免疫组织化学法观察APP蛋白在大鼠海马CA3区中的表达。结果:Morris水迷宫实验结显示:AD大鼠组的隐匿平台逃避潜伏期延长(P<0.05),在探索实验中,AD大鼠存在记忆障碍(P<0.05),而脑灵汤治疗后可使AD大鼠的逃避潜伏期缩短(P<0.05),大鼠平均探索次数明显增多(P<0.05)。AD组大鼠海马CA3区锥体细胞排列紊乱,有细胞缺失的现象,APP蛋白表达增多,脑灵汤治疗后可改善CA3区椎体细胞的病理改变以及降低APP蛋白的表达。结论:脑灵汤能明显改善AD模型大鼠低下的空间学习记忆能力,降低模型大鼠海马CA3区APP表达,对老年性痴呆大鼠学习记忆能力可能有提高作用。更多还原

【Abstract】 Objective To observe the effect of Naoling decoction on hippocamal histomorphology and the expression of amyloid precursor protein(APP) in CA3 region in rats with Alzheimer’s disease(AD),and to explore the therapeutical and the potential mechanism.Methods Forty SD rats were classified into 5 groups: a normal group,a sham-operated group,an AD group,a Naoling decoction group,and a Naofukang group.Alzheimer’s disease model was established by Aβ1-42 injected into the hippocamal in the rats.The faculty of learning and memory was evaluated by Morris water maze.The changes of cell morphology were detected by HE staining.Expression of APP in CA3 region was mea-sured with immunohistochemical staining.Results Morris water maze experiment showed that the escape latency of hidden platform in the AD group were delayed significantly(P<0.05) and the average times of passing was decreased(P<0.05).In the hippocampal CA3 field of the AD rats,HE staining and immunohistochemical test showed that pyramidal cells disturbed,neurons decreased significantly and expression of APP protein increased(P<0.05).Naoling decoction treatment improved pyramidal cellular disorders and decreased APP expression.Conclusion Naoling decoction can distinctly improve the learning and memory ability,and reduce the expression of APP in the AD model rats,suggesting that Naoling decoction can be used for the treatment of AD in rats.更多还原