【摘要】 目的:探讨胃癌患者血管内皮生长因子C和D(vascular endothelial growth factor-Cand-D,VEGF-CandVEGF-D)在胃癌组织中的表达及其与肿瘤淋巴结转移的关系。方法:应用免疫组织化学SABC法及Real-timePCR法检测32例胃癌组织和32例正常胃组织中VEGF-C,VEGF-D蛋白及其mRNA的表达。结果:胃癌组织VEGF-C和VEGF-D表达明显高于正常胃组织(P<0.01),其中淋巴结转移组VEGF-C,VEGF-D的表达与淋巴结未转移组间差异有统计学意义(P<0.05)。VEGF-C和VEGF-D在胃癌组织中的表达与淋巴结转移有关(P<0.01)。VEGF-C和VEGF-D的表达在高分化癌、中分化癌和低分化癌中的差别有统计学意义(P<0.05)。VEGF-C和VEGF-D在胃癌细胞中的表达与患者年龄、性别和淋巴结远处转移无关(P>0.05)。结论:胃癌细胞非摄入性高表达的VEGF-C和VEGF-D与淋巴结转移密切相关,可作为评估胃癌患者预后的重要参考指标。更多还原

【Abstract】 Objective To explore the expression of vascular endothelial growth factor (VEGF) C and D in gastric cancer and its relationship with tumor angiogenesis and lymph node metastasis.Methods Immunohistochemistry(SABC) and real-time PCR were used to detect the expression of VEGF-C,VEGF-D protein and mRNA in 32 gastric cancer tissues and 32 normal gastric tissues.Results The positive expression rate of VEGF-C and VEGF-D in gastric cancer tissue was significantly higher than that of normal gastric tissues (P<0.01),the expression of VEGF-C and VEGF-D in the gastric cancer group and the lymph node metastasis group were significantly different (P<0.05).The expression of VEGF-C and VEGF-D in gastric carcinoma was positively correlated with lymph node metastasis (P<0.01),the expression of VEGF-C and VEGF-D in well-differentiated carcinoma,moderately differentiated carcinoma and poorly differentiated carcinoma was statistically different (P<0.05).VEGF-C and VEGF-D expressions in gastric cancer cells were not related to the patient’s age,sex,and lymph node distant metastasis (P>0.05).Conclusion The non-intake high expression of VEGF-C and VEGF-D in gastric cancer cells is closely related to lymph node metastasis.They serve as the important reference indicator to assess the prognosis in gastric cancer patients.更多还原