【摘要】 测定竹黄无性型菌株ZHLS-03发酵菌丝体中竹红菌甲素的含量,并对其菌丝提取物的清除自由基活性和抑菌活性进行了研究。按50μg/ml的浓度测定了菌丝体石油醚、乙酸乙酯和甲醇等3种提取物对水稻纹枯病菌、稻瘟病菌、棉花枯萎病菌3种植物病原菌以及白色假丝酵母、金黄色葡萄球菌2种病原细菌的抑制能力;同时测定菌丝体的石油醚、乙酸乙酯和饱和正丁醇等3种提取物对DPPH自由基的清除率。结果表明:菌株ZHLS-03液体培养至6d,菌丝体中竹红菌甲素含量最高,可达到2.8029mg/g;菌丝体甲醇和正丁醇提取物分别具有较强的抑菌活性和清除自由基活性,甲醇相对棉花枯萎病菌的抑制率为74.78%,抗白色假丝酵母的抑菌圈直径达到20.69mm,且反应15min后正丁醇提取物对DPPH自由基的清除率可达93.24%。更多还原

【Abstract】 The hypocrellin A content of ZHLS-03 mycelium was analyzed,and the free radical scavenging rate and antimicrobial activity of ZHLS-03 strain extracts were also researched.The antimicrobial activities of petroleum ether,ethyl acetate and methanol extracts were detected against Rhizoctonia solani,Pyricularia grisea,Fusarium oxysporum f.sp.vasinfectum,Candida albicans and Staphylococcus aureus with antimicrobial assay models,and the free radical scavenging activities against DPPH of petroleum ether,ethyl acetate and n-butanol extracts were also detected.The results showed that the content of mycelium hypocrellin A in the mucelium reached the highest value of 2.8029mg/g while cultured for 6 days.The bioactive tests showed that both methanol extract and N-butanol extract of mycelium had high inhibition activities and free radical scavenging activities.The Fusarium oxysporum f.sp.vasinfectum inhibitions ratio of methanol extract was 74.78%,and the diameter of Candida albicans inhibition ring was 20.69mm The DPPH free radical scavenging activity of N-butanol extract reached 93.24% at the measurement time of 15min.更多还原