节点文献
1种新整体透明技术的研究(英文)
A New Technique for Whole Clearing
【摘要】 [目的]研究1种新整体透明与制片技术。[方法]将酒精溶液保存的千金藤幼嫩种子和高粱新鲜的雌蕊与珠心分离出来后,用84消毒液溶液对其进行浸泡、透明,然后按照3种制片方法进行制片处理和封片。①将材料取出放在凹玻片的凹槽内,在材料表面滴上数滴84消毒液,封片后即可进行观察。②将幼嫩种子从84消毒液中取出,放入盛有清水的小称量瓶中浸泡数分钟,然后重新换上干净的清水进行浸泡。重复漂洗数次后,将幼嫩种子放入盛有栀子色素溶液的小称量瓶中浸泡染色。当幼嫩种子被染上较深颜色时,将其取出放在凹玻片的凹槽内,滴数滴清水或栀子色素溶液,封片后即可进行观察。③将材料取出放在凹玻片的凹槽内,在材料表面滴1至数滴无水乙醇和油镜油混合液(约100∶1),并将扩散到周围的白色液体用吸水纸吸去,待混合液挥发将尽,材料完全暴露在空气中时,再次滴上数滴无水乙醇和油镜油混合液。重复2~4次后,滴数滴上述混合液,封片后即可进行观察。若要制成永久制片,则在混合液挥发将尽时,在植物材料上滴数滴油镜油后封片。观察后,可长时间保存。[结果]在普通显微镜下不仅能观察到千金藤幼嫩种子中不同发育时期的球形胚和心型胚,还能观察到高粱雌蕊的特征、珠心表面的珠心突、胚囊和承珠盘;在荧光显微镜可观察到高粱株心的胚囊和承珠盘的荧光图像。[结论]使用84消毒液作为整体透明技术的透明剂,不仅能观察保存后颜色变深或新鲜的植物材料,还能简化制片过程、缩短制片时间。
【Abstract】 [Objective] The research aimed to study a new whole clearing and preparation technology.[Method] After young seeds of Stephania sp.preserved in the alcohol solution,as well as the fresh pistil and nucellus of Sorghum bicolor were separated,they were soaked and cleared in the solution of 84 disinfectant.And the plant materials could be mounted by using three methods.[Result] The globular embryos and heart-shaped embryos at different stages of Stephania sp.as well as the pistil,nucellus,embryo sac,hypostase of Sorghum bicolor could be observed under a microscope.And the embryo sac and hypostase of Sorghum bicolor could be observed under a fluorescence microscope.[Conclusion] As the disinfectant of whole clearing technology,84 disinfectant could not only observe the dark preserved or fresh plant materials,but also simplify the preparation process and shorten the preparation time.
【Key words】 Whole clearing technology; 84 disinfectant; Seed; Pistil; Nucellus;
- 【文献出处】 Agricultural Science & Technology ,农业科学与技术(英文版) , 编辑部邮箱 ,2010年02期
- 【分类号】S-3
- 【被引频次】1
- 【下载频次】70