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反相高效液相色谱法测定培养基中苯磺隆
Determination of Tibenuron-Methyl in the Medium by Reversed-Phase High Performance Liquid Chromatography
【摘要】 采用反相高效液相色谱法,对农药苯磺隆在MS培养基中含量变化进行分析,使用C18和紫外检测器,样品前处理采用二氯甲烷萃取后旋蒸甲醇溶解,苯磺隆质量浓度在25~200μg/mL之间有良好的相关系数(R2=0.999).培养基苯磺隆样品溶液标准偏差0.082,变异系数0.16%,平均回收率100.86%,能够准确测得培养基中苯磺隆含量变化.
【Abstract】 A method is developed for the quantitative analysis of tribenuron-methyl by reversed-phase high performance liquid chromatography,in MS medium,on C18 Column with uv-absobance detector.The samples of ahead processing design adopt dichloromethane extraction and revolving evaporator and methanol dissolve.The linear relation was very good in the range of 25~200 μg/mL(R2=0.999).The samples standard deviation was 0.082,the coefficients of variation was 0.16 %,and the average recovery was 100.86 %.This method can accurately determinate tribenuron-methyl content in the medium.
- 【文献出处】 河南科学 ,Henan Science , 编辑部邮箱 ,2010年11期
- 【分类号】S482.4
- 【被引频次】1
- 【下载频次】70