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反相高效液相色谱法测定培养基中苯磺隆

Determination of Tibenuron-Methyl in the Medium by Reversed-Phase High Performance Liquid Chromatography

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【作者】 刘丽杜迅安明理赵明陈国参

【Author】 Liu Li1,2,Du Xun1,2,An Mingli1,Zhao Ming3,Chen Guocan1,2(1.Institute of Biology Co.,Ltd.,Henan Academy of Sciences,Zhengzhou 450008,China;2.Key Laboratory of Microbial Engineering of Henan Province,Zhengzhou 450008,China;3.School of Biological Engineering,Zhengzhou University,Zhengzhou 450000,China)

【机构】 河南省科学院生物研究所有限责任公司河南省微生物工程重点实验室郑州大学生物工程系

【摘要】 采用反相高效液相色谱法,对农药苯磺隆在MS培养基中含量变化进行分析,使用C18和紫外检测器,样品前处理采用二氯甲烷萃取后旋蒸甲醇溶解,苯磺隆质量浓度在25~200μg/mL之间有良好的相关系数(R2=0.999).培养基苯磺隆样品溶液标准偏差0.082,变异系数0.16%,平均回收率100.86%,能够准确测得培养基中苯磺隆含量变化.

【Abstract】 A method is developed for the quantitative analysis of tribenuron-methyl by reversed-phase high performance liquid chromatography,in MS medium,on C18 Column with uv-absobance detector.The samples of ahead processing design adopt dichloromethane extraction and revolving evaporator and methanol dissolve.The linear relation was very good in the range of 25~200 μg/mL(R2=0.999).The samples standard deviation was 0.082,the coefficients of variation was 0.16 %,and the average recovery was 100.86 %.This method can accurately determinate tribenuron-methyl content in the medium.

【关键词】 高效液相色谱苯磺隆测定培养基
【Key words】 HPLCtribenuron-methyl determinationmedium
【基金】 河南省重大公益科研项目(091100910500)
  • 【分类号】S482.4
  • 【被引频次】1
  • 【下载频次】70
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