【摘要】 为建立圆山药的离体培养体系,将野生苗盆栽后,切取其嫩叶、茎段、地下块茎和不定根作为外植体,探讨不同消毒方式的影响及不同生长调节剂组合对愈伤组织诱导、愈伤组织分化、不定芽诱导及腋芽增殖的影响。结果表明:①腋芽增殖途径的培养基配方为MS+BA3.0 mg/L+NAA 0.2 mg/L(增殖系数为3.1);②适宜的愈伤组织诱导培养基为MS+2,4—D 4 mg/L+6—BA 0.5 mg/L,其中以带腋芽的茎段为外植体诱导率较高,可达85.7%。BA浓度为4.0~6.0 mg/L时,不定芽的形成率最高,达97.5%;③增加BA和琼脂的浓度利于防止圆山药的褐化。更多还原

【Abstract】 An in vitro protocol was developed for multiplication of Dioscorea rotundata by using leaves,stem,tuber and adventitious root derived from plants grown in pot as explants.Effects of different plant growth regulators on callus induction,callus differentiation,adventitious bud induction and axillary bud multiplication were studied.The results showed that the optimum medium for proliferation of axillary bud located below the shoot tip was MS+BA 3.0mg/L+NAA 0.2mg/L(3.1 for coefficient of multiplication).The optimum medium for callus induction was MS+2,4-D 4mg/L+6-BA 0.5mg/L,and the rate of callus inducement was as high as 85.7% when stems with axillary buds were used as explants.When the concentration of BA was 4.0~6.0mg/L,the rate of adventitious bud formation could up to 97.5%.Increasing the concentration of BA and agar could decrease browning during the culture.更多还原